Origins of the avian neural crest: the role of neural plate-epidermal interactions.
Origins of the avian neural crest: the role of neural plate-epidermal interactions.
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DOI：
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发表时间：
1995-02
期刊：
影响因子：
4.6
通讯作者：
M. Selleck;M. Bronner‐Fraser
中科院分区：
文献类型：
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作者：
M. Selleck;M. Bronner‐Fraser
我们已经调查了谱系和组织的相互作用，导致鸟类神经嵴细胞形成的外胚层。假定的神经板移植到非神经外胚层在整个胚胎培养的组织相互作用的神经嵴的个体发育的作用。我们的研究结果表明，非神经外胚层和假定的神经板的并列诱导神经嵴细胞的形成。鹌鹑/小鸡重组表明，预期的神经板和预期的表皮可以有助于神经嵴。当在组织培养中进行类似的神经板/表皮对抗以观察神经嵴衍生物的形成时，表皮与早期（阶段4-5）或晚期（阶段6-10）神经板的并置导致黑素细胞和交感肾上腺细胞的产生。有趣的是，从早期阶段分离的神经板没有形成神经嵴细胞，而那些分离出来的黑素细胞，但不是脊衍生的交感肾上腺细胞。进行单细胞谱系分析，以确定神经嵴谱系从表皮谱系分化的时间，并阐明正常发育过程中神经板/表皮相互作用的时间。我们从阶段8至10+胚胎的结果显示，神经板/神经嵴谱系在神经管闭合时从表皮分离，表明神经诱导在开放神经板阶段仍在进行中。
We have investigated the lineage and tissue interactions that result in avian neural crest cell formation from the ectoderm. Presumptive neural plate was grafted adjacent to non-neural ectoderm in whole embryo culture to examine the role of tissue interactions in ontogeny of the neural crest. Our results show that juxtaposition of non-neural ectoderm and presumptive neural plate induces the formation of neural crest cells. Quail/chick recombinations demonstrate that both the prospective neural plate and the prospective epidermis can contribute to the neural crest. When similar neural plate/epidermal confrontations are performed in tissue culture to look at the formation of neural crest derivatives, juxtaposition of epidermis with either early (stages 4-5) or later (stages 6-10) neural plate results in the generation of both melanocytes and sympathoadrenal cells. Interestingly, neural plates isolated from early stages form no neural crest cells, whereas those isolated later give rise to melanocytes but not crest-derived sympathoadrenal cells. Single cell lineage analysis was performed to determine the time at which the neural crest lineage diverges from the epidermal lineage and to elucidate the timing of neural plate/epidermis interactions during normal development. Our results from stage 8 to 10+ embryos show that the neural plate/neural crest lineage segregates from the epidermis around the time of neural tube closure, suggesting that neural induction is still underway at open neural plate stages.