ISOLATION OF A PLURIPOTENT CELL-LINE FROM EARLY MOUSE EMBRYOS CULTURED IN MEDIUM CONDITIONED BY TERATOCARCINOMA STEM-CELLS
ISOLATION OF A PLURIPOTENT CELL-LINE FROM EARLY MOUSE EMBRYOS CULTURED IN MEDIUM CONDITIONED BY TERATOCARCINOMA STEM-CELLS
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DOI：
10.1073/pnas.78.12.7634
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
MARTIN, GR
中科院分区：
文献类型：
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作者：
MARTIN, GR
描述了直接从正常的植入前小鼠胚胎建立一种细胞系，当注射到小鼠体内时会形成畸胎癌。从分离的单个细胞衍生的亚克隆培养可以分化为多种类型的细胞，这一观察最终证明了这些胚胎干细胞的多能性。这种胚胎干细胞是从晚期囊胚的内细胞团中分离出来的，培养在已建立的畸胎癌细胞系[PSA-1]的条件下。这种条件培养液可能含有一种促进正常多能胚胎细胞增殖和/或抑制其分化的生长因子。这种获得胚胎干细胞的方法使从各种类型的非近交系胚胎(包括那些携带突变基因的胚胎)中分离出多能细胞系成为可能。这种细胞系的出现应该会使研究早期哺乳动物发育的新方法成为可能。
The establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice is described. The pluripotency of these embryonic stem cells was demonstrated conclusively by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types. Such embryonic stem cells were isolated from inner cell masses of late blastocysts cultured in medium conditioned by an established teratocarcinoma stem cell line [PSA-1]. Such conditioned medium might contain a growth factor that stimulates the proliferation and/or inhibits the differentiation of normal pluripotent embryonic cells. This method of obtaining embryonic stem cells makes feasible the isolation of pluripotent cells lines from various types of noninbred embryo, including those carrying mutant genes. The availability of such cell lines should make possible new approaches to the study of early mammalian development.