T cell chemotaxis in a simple microfluidic device
T cell chemotaxis in a simple microfluidic device
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DOI：
10.1039/b607071j
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发表时间：
2006-01-01
期刊：
影响因子：
6.1
通讯作者：
Butcher, Eugene C.
中科院分区：
文献类型：
--
作者：
Lin, Francis;Butcher, Eugene C.
本文介绍了使用一个简单的微流控装置研究T细胞趋化性。微流控装置使用软光刻在聚（二甲基硅氧烷）（PDMS）中制造，并且由“Y”型流体通道组成。通过注射泵将溶液注入装置中，并通过扩散在通道中产生浓度梯度。我们表明，实验测得的梯度配置文件同意很好的理论预测和梯度是稳定的细胞迁移的观察区域。使用该装置，我们证明了人类T细胞对趋化因子CCL19和CXCL12的单一和竞争梯度的强烈趋化性。由于该设备的简单性，它可以灵活地控制空间和时间上的梯度生成，并允许在单个芯片中生成多个梯度条件，以进行高度并行的趋化性实验。T细胞趋化性的可视化以前仅限于在3D基质中或在琼脂糖测定下的研究，其不允许条件的精确控制或变化。认识到淋巴细胞归巢在适应性免疫应答中的重要性，在微流体装置中研究T细胞趋化性的能力为研究淋巴细胞迁移和体外趋化性提供了一种新的方法。
This paper describes the use of a simple microfluidic device for studying T cell chemotaxis. The microfluidic device is fabricated in poly( dimethylsiloxane) ( PDMS) using soft- lithography and consists of a "Y'' type fluidic channel. Solutions are infused into the device by syringe pumps and generate a concentration gradient in the channel by diffusion. We show that the experimentally measured gradient profiles agree nicely with theoretical predictions and the gradient is stable in the observation region for cell migration. Using this device, we demonstrate robust chemotaxis of human T cells in response to single and competing gradients of chemokine CCL19 and CXCL12. Because of the simplicity of the device, it can flexibly control gradient generation in space and time, and would allow generation of multiple gradient conditions in a single chip for highly parallel chemotaxis experimentation. Visualization of T cell chemotaxis has previously been limited to studies in 3D matrices or under agarose assays, which do not allow precise control or variation in conditions. Acknowledging the importance of lymphocyte homing in the adaptive immune response, the ability to study T cell chemotaxis in microfluidic devices offers a new approach for investigating lymphocyte migration and chemotaxis in vitro.