CD40L-null NKT cells provide B cell help for specific antibody responses.
CD40L-null NKT cells provide B cell help for specific antibody responses.
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CD40L 缺失的 NKT 细胞为特异性抗体反应提供 B 细胞帮助。
DOI：
10.1016/j.vaccine.2011.09.060
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发表时间：
2011
期刊：
影响因子：
5.5
通讯作者：
Lang,MarkL
中科院分区：
文献类型：
--
作者：
Shah,HemangiB;Joshi,SunilK;Lang,MarkL
CD 1d结合糖脂对T依赖性抗体应答发挥有效的佐剂作用。这些机制包括表达CD 1d的B细胞和表达TCR的I型CD 1d限制性自然杀伤T细胞（NKT）之间的同源相互作用。然而，刺激B细胞的关键NKT衍生因子知之甚少。我们检验了CD 1d驱动的NKT细胞表达CD 40 L影响体液免疫的假设。在测量Ab应答之前，用Ag + CD 1d配体免疫含有CD 40 L +/+或CD 40 L −/−NKT细胞的骨髓嵌合体。CD 40 L −/−NKT细胞比表达CD 40 L的对照刺激更高的终点Ab滴度。相反，免疫CD 40 L −/−小鼠发现，当Th细胞缺乏CD 40 L时，CD 40 L −/−NKT细胞不能提供B细胞帮助。我们报告说，CD 40 L −/−NKT细胞可以提供帮助抗体的生产，并与CD 40 L +/+Th细胞合作。我们认为NKT细胞提供B细胞帮助的方式不同于Th细胞。
CD1d-binding glycolipids exert potent adjuvant effects on T-dependent Ab responses. The mechanisms include cognate interaction between CD1d-expressing B cells and TCR-expressing Type I CD1d-restricted natural killer T cells (NKTs). However, the critical NKT-derived factors that stimulate B cells are poorly understood. We tested the hypothesis that CD1d-driven CD40L expression by NKT cells influences humoral immunity. Bone marrow chimeras with CD40L+/+or CD40L−/−NKT cells were immunized with Ag plus CD1d ligand before measuring Ab responses. CD40L−/−NKT cells stimulated higher endpoint Ab titers than controls expressing CD40L. In contrast, immunization of CD40L−/−mice revealed that CD40L−/−NKT cells could not provide B cell help when Th cells lacked CD40L. We report that CD40L−/−NKT cells can provide help for Ab production and do so cooperatively with CD40L+/+Th cells. We suggest that the manner in which NKT cells provide B cell help is distinct from that of Th cells.