Ferulic acid inhibits H2O2-induced oxidative stress and inflammation in rat vascular smooth muscle cells via inhibition of the NADPH oxidase and NF-κB pathway
Ferulic acid inhibits H2O2-induced oxidative stress and inflammation in rat vascular smooth muscle cells via inhibition of the NADPH oxidase and NF-κB pathway
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DOI：
10.1016/j.intimp.2015.07.037
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发表时间：
2015-10-01
影响因子：
5.6
通讯作者：
He, Lang-chong
中科院分区：
文献类型：
--
作者：
Cao, Yan-jun;Zhang, Yan-min;He, Lang-chong
阿魏酸（FA）是一种膳食酚酸，具有广泛的治疗作用，包括抗衰老，抗肿瘤活性和抗高血压作用。本研究的目的是评估FA对细胞炎症和氧化应激的抑制作用，对过氧化氢（H2 O2）诱导的体外大鼠血管平滑肌细胞（VSMCs）损伤。在H2 O2孵育前2 h用FA预处理VSMCs。结果表明，FA通过降低MDA含量，提高SOD活性和GSH含量，抑制H2 O2引起的细胞损伤。FA可提高H2 O2暴露的大鼠VSMCs的存活率，恢复线粒体膜去极化。FA预处理可通过抑制NADPH氧化酶的表达，下调MAPK和AKT通路，从而降低ROS的产生。我们发现H2 O2刺激IL-6、IL-1 β、TNF-α的产生。FA预处理可通过抑制p-NF-κ B B和iNOS的表达而降低NO的生成。总之，我们的研究结果表明，FA可以作为一种新的药物，在这些病理治疗抑制NADPH氧化酶和NF-κ B，随后减少VSMC氧化应激和炎症。提示FA抑制VSMC炎症反应和氧化应激的作用可能与抑制NADPH和NF-κ B的表达，减少ROS的产生，减少VSMC凋亡有关。(C)2015年由Elsevier B. V.出版
Ferulic acid (FA) is a dietary phenolic acid and has a wide range of therapeutic effects, including anti-aging, antitumor activity and antihypertensive effects. The aim of present study was to evaluate the inhibitory effects of FA on cell inflammation and oxidative stress against hydrogen peroxide (H2O2)-induced injury in rat vascular smooth muscle cells (VSMCs) in vitro. VSMCs were pretreated with FA 2 h before H2O2 incubation. The results suggested that FA inhibited H2O2-induced cell injury by reducing the MDA and increasing the SOD activity and GSH content. In rat VSMCs exposed to H2O2, FA increased the cell viability and restored the mitochondrial membrane depolarization. The level of ROS generation was reduced by pretreatment with FA through inhibiting the expression of NADPH oxidase and down-regulating MAPK and AKT pathways. We found that H2O2 stimulated the production of IL-6, IL-1 beta, TNF-alpha. and NO, which could be reduced by pretreatment with FA through inhibiting the p-NF-kappa B as well as the iNOS expression. In conclusion, our results show that FA may serve as a novel drug in the treatment of these pathologies by inhibiting NADPH oxidase and NF-kappa B and subsequently decreasing VSMC oxidative stress and inflammation. These suggest that the inhibitory effect of FA on VSMC inflammation and oxidative stress is partially attributed to depressing NADPH and NF-kappa B expressions in VSMCs, decreasing the ROS production and reducing apoptosis of VSMCs. (C) 2015 Published by Elsevier B.V.