Structure and tissue-specific developmental expression of a sea urchin arylsulfatase gene.
Structure and tissue-specific developmental expression of a sea urchin arylsulfatase gene.
复制标题
海胆芳基硫酸酯酶基因的结构和组织特异性发育表达。
DOI：
10.1016/0012-1606(89)90157-7
复制
发表时间：
1989
影响因子：
2.7
通讯作者：
Angerer,RC
中科院分区：
文献类型：
--
作者：
Yang,Q;Angerer,LM;Angerer,RC
芳基磺化酶是一组能从多种底物（包括糖蛋白、类固醇和脑苷）中去除硫酸盐部分的酶。我们分离出了一个重组cDNA克隆，该克隆对应于一个编码purpuratus strongylocentrotus pluteus幼虫丰富蛋白的芳基硫酸盐酶（SpARS）信息。尽管脊椎动物芳基硫酸酯酶具有广泛的组织分布，但SpARS探针的原位杂交表明，海胆信息在胚胎中仅在体外外胚层及其前体的单一细胞类型中积累。该信息首先通过RNase保护试验在囊胚孵化期检测到，并通过pluteus幼虫期积累。cDNA克隆的开放阅读框长1701 nt，编码了一个预测分子量为61 kDa的推断蛋白。对相应基因组DNA克隆的分析表明，前mrna含有6个外显子。与芳基磺化酶活性在细胞外的事实一致，该多肽具有疏水先导序列和三个潜在的糖基化位点。此外，杂交表明，在囊胚中，芳基硫酸酯酶信息优先集中在细胞基侧的细胞核周围。帖前。purpuratus序列与最近报道的来自pulcherrimushemicentrotus的同一酶的序列非常相似，30%的氨基酸残基也与人类芳基硫酸酯酶C（类固醇硫酸酯酶）和芳基硫酸酯酶a的氨基酸残基相同。这四个mrna之间的序列关系表明，假设进化速度相同，分离人类基因的重复发生在棘皮动物和脊椎动物谱系分离的大约时间。
Arylsulfatases are a group of enzymes that remove sulfate moieties from a diverse set of substrates including glycoproteins, steroids, and cerebrosides. We have isolated recombinant cDNA clones corresponding to an arylsulfatase (SpARS) message that encodes an abundant protein of pluteus larvae of the sea urchinStrongylocentrotus purpuratus. Although vertebrate arylsulfatases have broad tissue distributions,in situhybridization with a probe for SpARS shows that the sea urchin message accumulates in the embryo only in the single cell type of aboral ectoderm and its precursors. The message is first detectable by RNase protection assays around hatching blastula stage and accumulates through pluteus larva stage. The open reading frame of cDNA clones is 1701 nt long and encodes a deduced protein with a predicted molecular mass of 61 kDa. Analysis of corresponding genomic DNA clones reveals that the pre-mRNA contains six exons. Consistent with the fact that arylsulfatase enzyme activity is extracellular, this polypeptide has a hydrophobic leader sequence and three potential glycosylation sites. Furthermore, hybridizationin situshows that in blastulae arylsulfatase message is preferentially concentrated around nuclei at the basal sides of cells. TheS. purpuratussequence is very similar to that recently reported for the same enzyme fromHemicentrotus pulcherrimusand 30% of the amino acid residues are also identical to those of both human arylsulfatase C (steroid sulfatase) and arylsulfatase A. Sequence relationships among these four mRNAs suggest that, assuming equal rates of evolution, the duplication separating the human genes occurred at about the time of separation of the echinoderm and vertebrate lineages.