The simultaneous differentiation of Potato virus Y strains including the newly described strain PVYNTN-NW by multiplex PCR assay
The simultaneous differentiation of Potato virus Y strains including the newly described strain PVYNTN-NW by multiplex PCR assay
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DOI：
10.1016/j.jviromet.2009.12.010
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发表时间：
2010-04-01
影响因子：
3.1
通讯作者：
Natsuaki, T.
中科院分区：
文献类型：
--
作者：
Ali, M. Chikh;Maoka, T.;Natsuaki, T.
新的PVY重组株和基因型分别命名为PVYNTN-NW和SYR-III，它们与PVYNTN和（PVYW）-W-N具有相同的特性。PVYNTN-NW在叙利亚的马铃薯田中占优势，并且能够诱导马铃薯块茎坏死环斑病（PTNRD）。由于PVY重组菌株的快速传播（可能是PVYNTN-NW的情况），特异性和可靠的检测方法是控制该菌株并最大限度地减少其传播的必要步骤。然而，PVYNTN-NW和SYR-III与PVYNTN和（PVYW）-W-N共有的性质使它们的鉴定复杂化，涉及多种检测方法。因此，开发了依赖于先前公布的和新设计的引物的组合的多重聚合酶链反应（PCR），用于检测和鉴定PVYNTN-NW和SYR-III在主要PVY菌株PVYO、PVYN、PVYNTN和（PVYW）-W-N的单一或混合感染中。此外，本发明的PCR测定能够检测P1区域中的重组点，从而能够区分重组菌株PVYNTN-NW、PVYNTN和（PVYW）-W-N的可变基因型。使用从叙利亚和日本收集的大量充分表征的PVY分离物（包括PVYNTN-NW、SYR-III、PVYO、NA-PVYN、（PVYW）-W-N和PVYNTN）证实了该PCR测定的可靠性。(C)2009 Elsevier B. V.保留所有权利。
New recombinant strain and genotype of PVY, designated as PVYNTN-NW and SYR-III, respectively, shared properties with PVYNTN and (PVYW)-W-N has been reported recently. PVYNTN-NW predominated in potato fields in Syria and was able to induce potato tuber necrotic ringspot disease (PTNRD). Due to the rapid spread of the recombinant strains of PVY which might be the case of PVYNTN-NW, a specific and reliable detection method is an essential step to control this strain and minimize its spread. The shared properties of PVYNTN-NW and SYR-III with PVYNTN and (PVYW)-W-N, however, complicate their identification involving multiple detection methods. Therefore, a multiplex polymerase chain reaction (PCR), that relies on a combination of previously published and newly designed primers was developed for the detection and identification of PVYNTN-NW and SYR-III in single or mixed infections with the main PVY strains, PVYO PVYN PVYNTN and (PVYW)-W-N. In addition, the present PCR assay was able to detect the recombination points in the P1 region enabling the differentiation of the variable genotypes of the recombinant strains PVYNTN-NW, PVYNTN and (PVYW)-W-N. The reliability of this PCR assay was confirmed using a significant number of well characterized PVY isolates collected from Syria and Japan including those of PVYNTN-NW, SYR-III, PVYO, NA-PVYN, (PVYW)-W-N and PVYNTN. (C) 2009 Elsevier B.V. All rights reserved.