Detection of mecA and ermA genes and simultaneous identification of Staphylococcus aureus using triplex real-time PCR from Malaysian S-aureus strain collections
Detection of mecA and ermA genes and simultaneous identification of Staphylococcus aureus using triplex real-time PCR from Malaysian S-aureus strain collections
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DOI：
10.1016/j.ijantimicag.2006.12.017
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发表时间：
2007-05-01
影响因子：
10.8
通讯作者：
Devi Sekaran, Shamala
中科院分区：
文献类型：
--
作者：
Shafiei Sabet, Negar;Subramaniam, Geetha;Devi Sekaran, Shamala
采用三重实时聚合酶链式反应(PCR)同时检测耐甲氧西林(MecA)、红霉素耐药(Erma)和金黄色葡萄球菌鉴定(FEMA)基因。临床分离的93株金黄色葡萄球菌中，耐甲氧西林金黄色葡萄球菌(MRSA)48株，甲氧西林敏感金黄色葡萄球菌(MSSA)45株。应用三重实时荧光定量聚合酶链式反应技术对所有MRSA菌株进行筛选，均检测到mecA、ermA和femA基因。三重实时荧光聚合酶链式反应在3h内完成，是检测耐药基因和鉴定金黄色葡萄球菌的一种有用的基因分型方法。这些发现将有助于临床实验室快速识别这些耐药基因和金黄色葡萄球菌，从而有利于患者的治疗。这项研究为研究人员研究局部耐药模式提供了宝贵的信息来源，这可以增加我们对抗生素耐药谱的了解，使用实时荧光聚合酶链式反应技术。(C)2007年Elsevier B.V.和国际化疗学会。版权所有。
A triplex real-time polymerase chain reaction (PCR) assay was used for the simultaneous detection of mecA (methicillin resistance), ermA (erythromycin resistance) and femA (Staphylococcus aureus identification) genes in a single assay. Among 93 clinical S. aureus hospital isolates, there were 48 methicillin-resistant S. aureus (MRSA) and 45 methicillin-sensitive S. aureus (MSSA) isolates. Screening the isolates using the triplex real-time PCR assay, the mecA, ermA and femA genes were detected in all MRSA isolates. The triplex real-time PCR assay was completed within 3 h and is a useful genotypic method for detecting the resistance determinants as well as for the identification of S. aureus isolates. These findings will assist the clinical laboratory in identifying these resistance genes and S. aureus rapidly, thus benefiting patient therapy. This study represents a valuable source of information for researchers to study the local antibiotic resistance pattern, which can increase our knowledge of the antibiotic resistance profile, using real-time PCR technology. (C) 2007 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.