NMR-STUDY OF INVIVO RIF-1 TUMORS - ANALYSIS OF PERCHLORIC-ACID EXTRACTS AND IDENTIFICATION OF H-1-RESONANCES, P-31-RESONANCES AND C-13-RESONANCES
NMR-STUDY OF INVIVO RIF-1 TUMORS - ANALYSIS OF PERCHLORIC-ACID EXTRACTS AND IDENTIFICATION OF H-1-RESONANCES, P-31-RESONANCES AND C-13-RESONANCES
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DOI：
10.1016/0167-4889(84)90042-9
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
GLICKSON, JD
中科院分区：
文献类型：
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作者：
EVANOCHKO, WT;SAKAI, TT;GLICKSON, JD
用多核核磁共振波谱和多种层析方法分析了辐射诱导纤维肉瘤(RIF-1)小鼠体内的高氯酸提取物。这一分析使得在体内观察到的31P共振被明确地指定为特定的含磷代谢物。在RIF-1肿瘤中发现，体内光谱中通常属于糖磷酸盐的区域主要含有磷酸乙醇胺和磷胆碱，而不是糖酵解中间产物。在这些在培养中生长的肿瘤细胞的提取物中以及在体内的光谱中都观察到了磷酸肌酸，这表明至少在体内观察到的一些磷酸肌酸来自肿瘤本身而不是来自正常组织。在高氯酸提取物的31P-核磁共振谱中，分辨了来自嘌呤和嘧啶核苷二磷酸和三磷酸的共振峰。核酸池的高效液相色谱分析表明，腺嘌呤衍生物是最丰富的成分，但其他核苷酸也有相当数量的存在。还对RIF-1提取物中的大部分代谢物进行了~1H和~(13)C共振归属。尤其重要的是观察乳酸的能力，乳酸水平可以提供体内和体外状态下这些细胞糖酵解的非侵入性测量。在肿瘤提取物中发现了高水平的氨基磺酸牛磺酸。
Perchloric acid extracts of radiation-induced fibrosarcoma (RIF-1) tumors grown in mice were analyzed by multinuclear NMR spectroscopy and by various chromatographic methods. This analysis has permitted the unambiguous assignment of the 31P resonances observed in vivo to specific P-containing metabolites. The region of the in vivo spectra generally assigned to sugar phosphates was found in RIF-1 tumors to contain primarily phosphorylethanolamine and phosphorylcholine rather than glycolytic intermediates. Phosphocreatine was observed in extracts of these tumor cells grown in culture as well as in the in vivo spectra, indicating that at least some of the phosphocreatine observed in vivo arises from the tumor itself and not from normal tissues. In the 31P-NMR spectra of the perchloric acid extract, resonances originating from purine and pyrimidine nucleoside di- and triphosphate were resolved. HPLC [high performance liquid chromatography] analyses of the nucleotide pool indicate that adenine derivatives were the most abundant components, but other nucleotides were present in significant amounts. The 1H and 13C resonance assignments of the majority of metabolites present in RIF-1 extracts were also made. Of particular importance is the ability to observe lactate, the levels of which may provide a noninvasive measure of glycolysis in these cells in both the in vivo and in vitro states. The aminosulfonic acid, taurine, was found in high levels in the tumor extracts.