De novo assembly and sex-specific transcriptome profiling in the sand fly Phlebotomus perniciosus (Diptera, Phlebotominae), a major Old World vector of Leishmania infantum.
De novo assembly and sex-specific transcriptome profiling in the sand fly Phlebotomus perniciosus (Diptera, Phlebotominae), a major Old World vector of Leishmania infantum.
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DOI：
10.1186/s12864-015-2088-x
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发表时间：
2015-10-23
期刊：
影响因子：
4.4
通讯作者：
Salvemini M
中科院分区：
文献类型：
--
作者：
Petrella V;Aceto S;Musacchia F;Colonna V;Robinson M;Benes V;Cicotti G;Bongiorno G;Gradoni L;Volf P;Salvemini M
白蛉白蛉（Phlebotomus perniciosus）是原生动物婴儿利什曼原虫（Leishmania infantum）的主要旧大陆媒介，婴儿利什曼原虫是人类和狗内脏和皮肤利什曼病的病原体，这是一种全球范围内重新出现的疾病，影响101个国家。尽管在过去的几年里，对这种白蛉物种的研究越来越感兴趣，但迄今为止，基因组资源的开发仍然有限。为了增加P. perniciosus的可用序列数据并开始研究白蛉性别分化的分子基础，我们对成年雄性和雌性进行了全转录组Illumina RNA测序（RNA-seq）并从头进行了转录组组装。我们收集了55，393个高质量的转录本，其中29，292个是独特的，从成年全身男性和女性池开始。11，736个转录物具有至少一个功能注释，包括全长低丰度唾液转录物，981个转录物被分类为推定的长非编码RNA，244个转录物编码推定的白蛉亚科特异性新蛋白。差异表达分析确定了8590成绩单显着偏向性别之间。其中，一些显示放松的选择性限制相比，他们的直系同源的新世界沙蝇物种Lutzomyia longipalpis。在本文中，我们提出了一个全面的转录组资源为白蛉物种P. perniciosus建立从短读RNA-seq和我们提供见解性别特异性基因表达在成年阶段。我们的分析代表了第一步的性别特异性基因和途径的识别和即将到来的调查这一重要的载体物种，包括性别偏见的基因和白蛉的性别分化的进化研究的基础。本文的在线版本（doi：10.1186/s12864-015-2088-x）包含补充材料，可供授权用户使用。
The phlebotomine sand fly Phlebotomus perniciosus (Diptera: Psychodidae, Phlebotominae) is a major Old World vector of the protozoan Leishmania infantum, the etiological agent of visceral and cutaneous leishmaniases in humans and dogs, a worldwide re-emerging diseases of great public health concern, affecting 101 countries. Despite the growing interest in the study of this sand fly species in the last years, the development of genomic resources has been limited so far. To increase the available sequence data for P. perniciosus and to start studying the molecular basis of the sexual differentiation in sand flies, we performed whole transcriptome Illumina RNA sequencing (RNA-seq) of adult males and females and de novo transcriptome assembly. We assembled 55,393 high quality transcripts, of which 29,292 were unique, starting from adult whole body male and female pools. 11,736 transcripts had at least one functional annotation, including full-length low abundance salivary transcripts, 981 transcripts were classified as putative long non-coding RNAs and 244 transcripts encoded for putative novel proteins specific of the Phlebotominae sub-family. Differential expression analysis identified 8590 transcripts significantly biased between sexes. Among them, some show relaxation of selective constraints when compared to their orthologs of the New World sand fly species Lutzomyia longipalpis. In this paper, we present a comprehensive transcriptome resource for the sand fly species P. perniciosus built from short-read RNA-seq and we provide insights into sex-specific gene expression at adult stage. Our analysis represents a first step towards the identification of sex-specific genes and pathways and a foundation for forthcoming investigations into this important vector species, including the study of the evolution of sex-biased genes and of the sexual differentiation in phlebotomine sand flies. The online version of this article (doi:10.1186/s12864-015-2088-x) contains supplementary material, which is available to authorized users.