Determination of genetic variability among the isolates of Metarhizium anisopliae var. anisopliae from different geographical origins
Determination of genetic variability among the isolates of Metarhizium anisopliae var. anisopliae from different geographical origins
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金龟子绿僵菌变种分离株遗传变异性的测定
DOI：
10.1007/s11274-010-0466-8
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发表时间：
2011-02-01
影响因子：
4.1
通讯作者：
Ren, Shun-Xiang
中科院分区：
文献类型：
--
作者：
Freed, Shoaib;Jin, Feng-Liang;Ren, Shun-Xiang
金龟子绿僵菌是一种高效的昆虫生防真菌。昆虫病原真菌金龟子绿僵菌的流行和遗传变异。对亚洲国家（中国、老挝、新加坡和韩国）和欧洲国家（荷兰）的绿僵菌进行了检查。该菌在中国农业和森林土壤中广泛分布，尤其是在南部和西南部地区，最高回收率为81.6%，而在老挝仅在森林土壤中发现大量真菌，荷兰的土壤似乎也是昆虫病原菌的良好来源，昆虫病原真菌的回收率也很高。SSR和ITS-rDNA序列分析结果表明，这两个菌株之间存在紧密连锁。62个菌株的基因多样性和多态信息含量平均值分别为0.37和0.63。大多数菌株属于密切相关的基因型之一，这些被发现是占主导地位的农业以及森林生态系统。不同地点和不同来源的分离株之间的遗传距离变化最小，范围为0.23%至0.34%，来自老挝和荷兰的分离株之间的遗传距离最大为0.34%。有限的变化的原因是不确定的，但是，即使是菌株之间的相似性指数可以赋予足够的知识的方法的影响，开发这种真菌作为微生物控制剂。
The entomopathogenic fungus Metarhizium anisopliae is currently used as an efficient biological control agent against different insects. The prevalence and genetic variability of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae in Asian countries (China, Laos, Singapore and South Korea) and a European country (The Netherlands) was examined. The fungus was found to be widespread in agricultural and forest soils throughout China especially in the south and south western regions, with a maximum recovery percentage of 81.6, while in Laos it was found to be abundant in the forest soils only, the soil of Netherlands also seemed to be an excellent source of entomopathogens with a significant recovery of insect pathogenic fungi. Simple sequence repeats (SSR) and ITS-rDNA sequence data showed that the isolates are closely linked to each other. The gene diversity (He) and polymorphic information content values of sixty two isolates showed mean values of 0.37 and 0.63. The majority of the isolates belonged to one of the closely related genotypes and these were found to be dominant in the agricultural as well as forest ecosystem. Genetic distances among the isolates according to locations and different sources showed minimal variation ranging from 0.23 to 0.34%, with maximum genetic distance of 0.34% among the isolates from Laos and The Netherlands. The reason for the limited variation is uncertain, however even the similarity index among the isolates can endow with sufficient knowledge for the implications of the methods to develop this fungus as a microbial control agent.