Covalent Functionalization of PMMA Surface with L-3,4-Dihydroxyphenylalanine (L-DOPA) to Enhance its Biocompatibility and Adhesion to Corneal Tissue
Covalent Functionalization of PMMA Surface with L-3,4-Dihydroxyphenylalanine (L-DOPA) to Enhance its Biocompatibility and Adhesion to Corneal Tissue
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DOI：
10.1002/admi.201900767
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发表时间：
2020-01-01
影响因子：
5.4
通讯作者：
Gonzalez-Andrades, Miguel
中科院分区：
文献类型：
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作者：
Sharifi, Roholah;Mahmoudzadeh, Soudabe;Gonzalez-Andrades, Miguel
波士顿角膜假体(B-KPro)是全球最常见的植入人工角膜，适用于患有严重角膜疾病的患者，特别是那些多次同种异体移植失败的患者。尽管提供了良好的视觉恢复，但聚甲基丙烯酸甲酯(PMMA)制成的干细胞与供体组织之间的粘附性较差构成了一个挑战，影响了B-KPro的临床结果。采用单分子共价键的方法，用L-3，4-二羟基苯丙氨酸(L-DOPA)对聚甲基丙烯酸甲酯进行表面官能化处理，研究了其化学、光学、力学和生物学性能。功能化过程显著提高了PMMA的生物相容性，而不影响其光学和机械性能。人角膜成纤维细胞(Hcf)和人角膜上皮细胞(HcEp)接种于L多巴表面，在7天的细胞培养中，与聚甲基丙烯酸甲酯相比，两者均表现出更大的融合和代谢率。与PMMA组相比，在L-DOPA上培养的HCF表达ALDH3A1、Ki67、整合素1和FAK，而不表达α-SMA，PMMA组表达更多的α-SMA。这表明L-多巴表面促进了细胞的黏附、增殖和迁移，而不会对细胞的表型产生不利影响。本研究提供了一种廉价、高效的策略，用L多巴对材料进行表面修饰，以实现医疗器械的最佳生物相容性和生物集成。
The Boston keratoprosthesis (B-KPro) is globally the most commonly implanted artificial cornea for patients with severe corneal diseases, particularly those with multiple allograft failures. Despite providing a good visual recovery, the poor adhesion between the poly(methyl methacrylate) (PMMA)-made stem and the donor tissue poses a challenge, impacting the clinical outcome of the B-KPro. Using single-molecule covalent bonding, PMMA surface is functionalized with l-3,4-dihydroxyphenylalanine (l-DOPA) and its chemical, optical, mechanical, and biological properties are studied. The functionalization process significantly improves biocompatibility of PMMA, without affecting its optical and mechanical properties. Human corneal fibroblasts (HCF) and human corneal epithelial cells (HCEp) seeded on l-DOPA surface both exhibit greater confluency and metabolic rate compared to those of PMMA during 7-day cell culture. Moreover, HCF cultured on l-DOPA demonstrates a higher expression of ALDH3A1, Ki67, Integrin 1, and FAK with no expression of alpha-SMA, compared to those of PMMA, which instead show greater expression of alpha-SMA. These suggest that l-DOPA surface fosters cellular adhesion, proliferation, and migration, without adversely impacting the phenotype of the cells. This study offers an inexpensive and efficient tactic to modify the surface of materials with l-DOPA to achieve the optimal biocompatibility and biointegration of medical devices.