Scanning concentration correlation spectroscopy using the confocal laser microscope.
Scanning concentration correlation spectroscopy using the confocal laser microscope.
复制标题
使用共焦激光显微镜扫描浓度相关光谱。
DOI：
10.1016/s0006-3495(94)80801-x
复制
发表时间：
1994
影响因子：
3.4
通讯作者：
Carson,JH
中科院分区：
文献类型：
--
作者：
Koppel,DE;Morgan,F;Cowan,AE;Carson,JH
浓度相关光谱学允许评估复杂系统中的分子运动。该技术通常通过荧光分子强度（荧光相关光谱）等方法监测浓度波动。我们在这里描述了使用扫描共聚焦激光显微镜来测量空间和时间上的相关函数。与传统方法相比，这种方法有两个主要优点。首先，从样本的不同区域收集数据可以显著提高信噪比。其次，利用高时空分辨率的相关方法分析胶体金的分子运动。利用mrc600激光扫描系统采集了768个独立子体的数据，并确定了时空相关函数。我们使用两种不同类型的样品，荧光标记的溶液中的DNA分子和胶体金标记的脂质在平面双分子层中演示了该技术。这种方法，我们称之为“扫描浓度相关光谱”，提供了一种使用现有仪器对分子运动进行高分辨率相关分析的直接方法。
Concentration correlation spectroscopy allows the assessment of molecular motions in complex systems. The technique generally monitors concentration fluctuations by means of some method such as the intensity of fluorescent molecules (fluorescence correlation spectroscopy). We describe here the use of scanning confocal laser microscopy to measure correlation functions in both space and time. This methodology offers two major advantages over conventional methods. First, collecting data from different regions of the sample significantly increases the signal-to-noise ratio. Second, molecular motions of colloidal gold can be analyzed by correlation methods with high temporal and spatial resolution. Using a MRC 600 laser scanning system, we collect data from an ensemble of 768 independent subvolumes and determine the space-time correlation function. We demonstrate the technique using two different types of samples, fluorescently labeled DNA molecules in solution and colloidal gold-tagged lipids in a planar bilayer. This approach, which we term "scanning concentration correlation spectroscopy," provides a straightforward means of performing high resolution correlation analysis of molecular motions with available instrumentation.