Characterization of neuropeptide Y in snakeskin gourami and the change in its expression due to feeding status and melanocortin 4 receptor expression
Characterization of neuropeptide Y in snakeskin gourami and the change in its expression due to feeding status and melanocortin 4 receptor expression
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DOI：
10.1016/j.ygcen.2012.07.024
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发表时间：
2012-11-01
影响因子：
2.7
通讯作者：
Yoshizaki, Goro
中科院分区：
文献类型：
--
作者：
Boonanuntanasarn, Surintorn;Jangprai, Araya;Yoshizaki, Goro
在这项研究中，我们的特点是神经肽Y（NPY）的mRNA在蛇皮丝足（Trichogaster pectoralis）（TpNPY）。TpNPY显示了先前报道的NPY的典型特征，并且它与其他脊椎动物的NPY蛋白具有高度的同源性。系统发育分析表明，TpNPY与棘形目和鲑形目鱼类中的NPY密切相关。通过实时RT-PCR和原位杂交评估，发现TpNPY在所有脑区中普遍表达。此外，在外周组织中观察到TpNPY的分级表达水平;例如，在鳃、肝、肾、胃、肠、脾和性腺中发现中等水平的TpNPY，而在肌肉中发现低水平的TpNPY。在不同的大脑区域，包括端脑，中脑，后脑和间脑的TpNPY的表达与日常饮食习惯的变化进行了研究。TpNPY的表达水平的波动，观察了24小时餐后期间。除了端脑，TpNPY的表达减少，发现餐后，而TpNPY的峰值水平观察到1小时前预定的早餐。此外，在整个昼夜摄食周期中，端脑和间脑中的TpNPY和TpMC 4 R之间存在正相关，这表明NPY和黑皮质素系统的功能之间存在联系，以调节日常摄食。将鱼脑与MC 4 R拮抗剂（即，HS 024），并测定TpNPY和TpMC 4 R的表达。有趣的是，在HS 024的作用下，TpNPY和TpMC 4 R的表达之间存在显著的关系，这表明MC 4 R和NPY之间存在相互作用，特别是在吞噬状态下。(C)2012 Elsevier Inc. All rights reserved.
In this study, we characterized the neuropeptide Y (NPY) mRNA in snakeskin gourami (Trichogaster pectoralis) (TpNPY). TpNPY displayed characteristics typical of previously reported NPYs, and it exhibited a high degree of homology with the NPY proteins of other vertebrates. A phylogenetic analysis demonstrated that TpNPY was closely related to the NPYs found in the acanthomorpha and salmoniformes fish species. TpNPY was found to be ubiquitously expressed in all brain regions when assessed by real-time RT-PCR and in situ hybridization. In addition, a graded expression level of TpNPY was observed in peripheral tissues; for example, a moderate level of TpNPY was found in the gills, liver, kidney, stomach, intestine, spleen and gonads, while a low level of TpNPY was found in the muscle. The change in expression of TpNPY with respect to daily feeding habits was investigated in distinct brain regions, including the telencephalon, mesencephalon, metencephalon, and diencephalon. Fluctuations in the expression level of TpNPY were observed for a 24 h post-prandial period. Except for the telencephalon, a reduction in TpNPY expression was found after a meal, while a peak level of TpNPY was observed 1 h before the scheduled breakfast. Furthermore, there was a positive correlation between TpNPY and TpMC4R in the telencephalon and diencephalon throughout the circadian feeding cycle, which suggests that there is a connection between the function of NPY and the melanocortin system for the regulation of daily feeding. Fish brains were incubated with an MC4R antagonist (i.e., HS024), and the expression of TpNPY and TpMC4R was measured. Interestingly, there was a significant relationship between the expression of TpNPY and TpMC4R under the effects of HS024, which demonstrates that there are interactions between MC4R and NPY, particularly in a hyperphagic state. (C) 2012 Elsevier Inc. All rights reserved.