HOMEOTIC TRANSFORMATIONS IN THE MOUSE INDUCED BY OVEREXPRESSION OF A HUMAN HOX3.3 TRANSGENE
HOMEOTIC TRANSFORMATIONS IN THE MOUSE INDUCED BY OVEREXPRESSION OF A HUMAN HOX3.3 TRANSGENE
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DOI：
10.1016/0092-8674(92)90387-r
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发表时间：
1992-12-11
期刊：
影响因子：
64.5
通讯作者：
DEROBERTIS, EM
中科院分区：
文献类型：
--
作者：
JEGALIAN, BG;DEROBERTIS, EM
产生携带40个拷贝的人Hox3.3基因的永久转基因小鼠系。由此产生的小鼠在胚胎的后部区域表达大量的Hox3.3蛋白，而这种同源结构域蛋白通常不表达。转基因导致骨骼的同源异型转化，特别是在腰部区域出现额外的一对肋骨，将后部肋骨的形状转化为更前部的肋骨，以及将额外的一对肋骨连接到胸骨。该品系的表型类似于通过Hox 3.1的靶向功能丧失突变获得的表型（Le Mouellic et al. 1992年）。在瞬时测定中，人Hox3.3转基因也导致在更后部椎骨中形成额外的肋骨。
A permanent transgenic mouse line was generated carrying 40 copies of the human Hox3.3 gene. The resulting mice express large amounts of Hox3.3 protein in posterior regions of the embryo where this homeodomain protein is normally not expressed. The transgene causes homeotic transformations of the skeleton, in particular the appearance of an extra pair of ribs in the lumbar region, transformation of the shape of posterior ribs into that of more anterior ones, and the joining of an additional pair of ribs to the sternum. The phenotype of this line resembles that obtained by the targeted loss-of-function mutation of Hox3.1 (Le Mouellic et al., 1992). In transient assays, the human Hox3.3 transgene leads to the formation of additional ribs in more posterior vertebrae as well.