Surface modification of liposomes by saccharides: vesicle size and stability of lactosyl liposomes studied by photon correlation spectroscopy.
Surface modification of liposomes by saccharides: vesicle size and stability of lactosyl liposomes studied by photon correlation spectroscopy.
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DOI：
10.1016/j.jcis.2005.03.088
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发表时间：
2005-09
影响因子：
9.9
通讯作者：
Junmin Zhu;F. Yan;Zhongwu Guo;R. Marchant
中科院分区：
文献类型：
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作者：
Junmin Zhu;F. Yan;Zhongwu Guo;R. Marchant
细胞糖萼是脂质体表面修饰的一个有吸引力的模型，因为它的水合寡糖层抑制非特异性蛋白质吸附，并可以提供对所需位点的特异性。在这里，我们报告使用乳糖作为模型糖修饰脂质体表面和检查囊泡的大小和稳定性。合成了以十八烷基和十八酰基为脂尾的乳糖基脂质，即乳糖基醚-脂质（6a）和乳糖基酯-脂质（6 b），并采用挤出法制备了它们的脂质体。在室温下，通过光子相关光谱（PCS）研究了糖脂结构、浓度和挤出膜孔径对囊泡大小和稳定性的影响。含有5或10 mol%乳糖基脂质的所有脂质体具有窄的尺寸分布，并且在室温下保持稳定至少一个月，这与1，2-二硬脂酰-sn-甘油-3-磷酸胆碱（DSPC）-和聚（乙二醇）（PEG）-脂质体相当。乳糖基酯-脂质体的最大掺入量为15摩尔%，而乳糖基醚-脂质体的最大掺入量仅为10摩尔%。乳糖基酯-脂质体具有更好的稳定性，并且在掺入15或20摩尔%的乳糖基脂质时比乳糖基醚-脂质体表现出更小的尺寸变化。这可能归因于乳糖酯-脂质与DSPC具有更好的结构相容性。PCS结果表明，糖脂结构和浓度是影响囊泡稳定性的主要因素，而挤出膜的孔径大小没有影响。
The cell glycocalyx is an attractive model for surface modification of liposomes, because its hydrated oligosaccharide layer inhibits nonspecific protein adsorption and can provide specificity towards desired sites. Here, we report on the use of lactose as a model saccharide to modify the liposome surface and examine the vesicle size and stability. Two kinds of lactosyl lipids, including lactosyl ether-lipid (6a) and lactosyl ester-lipid (6b), which contain octadecyl and octadecanoyl as the lipid tails, respectively, were synthesized and their liposomes were prepared by the extrusion method. The effects of glycolipid structure, concentration, and the pore size of the extrusion membrane on vesicle size and stability were investigated at room temperature by photon correlation spectroscopy (PCS). All liposomes with 5 or 10 mol% of lactosyl lipids had a narrow size distribution and remained stable at room temperature for at least one month, which is comparable to 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)- and poly(ethylene glycol) (PEG)-liposomes. The maximum incorporation of lactosyl ester-lipid into liposomes was 15 mol%, compared with only 10 mol% for the lactosyl ether-lipid. The lactosyl ester-liposomes had better stability and exhibited less size change than the lactosyl ether-liposomes at 15 or 20 mol% of lactosyl lipids incorporated. This may be attributed to the better structural compatibility of lactosyl ester-lipid with DSPC. The PCS results show that the glycolipid structure and concentrations are major factors that affect vesicle stability, while the pore size of extrusion membranes has no influence.