Which extractant should be used for the screening and isolation of antimicrobial components from plants?
Which extractant should be used for the screening and isolation of antimicrobial components from plants?
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DOI：
10.1016/s0378-8741(97)00123-2
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发表时间：
1998-02-01
影响因子：
5.4
通讯作者：
Eloff, JN
中科院分区：
文献类型：
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作者：
Eloff, JN
用丙酮、乙醇、甲醇、二氯甲烷、甲醇/氯仿/水和水按1:10的比例提取了两种已知具有抗菌活性的植物Anthocleista granflora和Combretum erythrophyllum的冷冻干燥和细磨叶片。对提取的化合物的数量和多样性、提取的抑制剂数量、提取速率、生物测定毒性、溶剂去除难易程度和生物危害进行了评价。建立了一个任意评分系统来评价不同萃取剂的上述参数。丙酮对这些植物的处理效果最好，其任意值为102，其次是甲醇/氯仿/水（81）、二氯甲烷（79）、甲醇（71）、乙醇（58）和水（47）。四次五分钟的连续提取，极细研磨的桔梗以高速率摇提，提取了97%的总抗菌活性。(C) 1998爱思唯尔科学爱尔兰有限公司
Freeze dried and finely ground leaves of two plants with known antimicrobial activity, Anthocleista grandiflora and Combretum erythrophyllum were extracted with acetone, ethanol, methanol, methylenedichloride, methanol/chloroform/water and water at a 1 to 10 ratio in each case. The quantity and diversity of compounds extracted, number of inhibitors extracted, rate of extraction, toxicity in a bioassay, ease of removal of solvent and biological hazard were evaluated for each extractant. An arbitrary scoring system was developed to evaluate the above parameters for the different extractants. Acetone gave the best results with these plants with an arbitrary value of 102 followed by methanol/chloroform/water (81), methylene dichloride (79), methanol (71), ethanol (58) and water (47). Four five minute sequential extractions of very finely ground A. grandiflora shaking at a high rate extracted 97% of the total antimicrobial activity. (C) 1998 Elsevier Science Ireland Ltd.