Conservation of two distinct types of 100S ribosome in bacteria
Conservation of two distinct types of 100S ribosome in bacteria
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DOI：
10.1111/gtc.12057
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发表时间：
2013-07-01
期刊：
影响因子：
2.1
通讯作者：
Wada, Akira
中科院分区：
文献类型：
--
作者：
Ueta, Masami;Wada, Chieko;Wada, Akira
在细菌中，70 S核糖体（由30 S和50 S亚基组成）二聚化形成100 S核糖体，这首先在大肠杆菌中发现。核糖体调节因子（RMF）和冬眠促进因子（HPF）介导这种二聚化在稳定期。100 S核糖体在饥饿条件下是无活性的，但从饥饿状态转移到新鲜培养基中后，100 S核糖体解离成两个有活性的70 S核糖体。因此，100 S核糖体被称为“冬眠核糖体”。编码RMF的基因广泛存在于γ蛋白菌纲中，但不存在于任何其他细菌中。在这项研究中，100 S核糖体的形成在6种γ-变形菌和8种属于其他细菌类进行了比较。这两组细菌之间有几个显著的差异：（i）在γ-变形菌中，100 S核糖体的形成由RMF和短HPF介导，类似于大肠杆菌，而在其他细菌中，100 S核糖体的形成仅由长HPF介导;（ii）RMF/短HPF介导的100 S核糖体形成特异性地发生在稳定期，而长HPF介导的100 S核糖体的形成发生在所有的生长阶段;和（iii）长HPF形成的100 S核糖体比RMF和短HPF形成的100 S核糖体更稳定。
In bacteria, 70S ribosomes (consisting of 30S and 50S subunits) dimerize to form 100S ribosomes, which were first discovered in Escherichia coli. Ribosome modulation factor (RMF) and hibernation promoting factor (HPF) mediate this dimerization in stationary phase. The 100S ribosome is translationally inactive, but it dissociates into two translationally active 70S ribosomes after transfer from starvation to fresh medium. Therefore, the 100S ribosome is called the hibernating ribosome'. The gene encoding RMF is found widely throughout the Gammaproteobacteria class, but is not present in any other bacteria. In this study, 100S ribosome formation in six species of Gammaproteobacteria and eight species belonging to other bacterial classes was compared. There were several marked differences between the two groups: (i) Formation of 100S ribosomes was mediated by RMF and short HPF in Gammaproteobacteria species, similar to E.coli, whereas it was mediated only by long HPF in the other bacterial species; (ii) RMF/short HPF-mediated 100S ribosome formation occurred specifically in stationary phase, whereas long HPF-mediated 100S ribosome formation occurred in all growth phases; and (iii) 100S ribosomes formed by long HPF were much more stable than those formed by RMF and short HPF.