Antiproliferative effect of exemestane in lung cancer cells.
Antiproliferative effect of exemestane in lung cancer cells.
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DOI：
10.1186/1476-4598-8-109
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发表时间：
2009-11-24
期刊：
影响因子：
37.3
通讯作者：
Kalofonos H
中科院分区：
文献类型：
--
作者：
Koutras A;Giannopoulou E;Kritikou I;Antonacopoulou A;Evans TR;Papavassiliou AG;Kalofonos H
最近的证据表明，雌激素信号可能参与了非小细胞肺癌（NSCLC）的发病机制。芳香化酶是一种酶复合物，催化雌激素合成的最后一步，存在于包括肺在内的几种组织中。在目前的研究中，我们研究了芳香酶抑制剂阿司美坦在人NSCLC细胞系H23和A549中的活性。在两种细胞系中均检测到芳香化酶表达。与A549细胞相比，H23细胞显示较低的芳香化酶蛋白和mRNA水平。依西美坦降低细胞增殖和增加细胞凋亡在两个细胞系，48小时后，其应用，与A549表现出更高的敏感性比H23细胞。芳香化酶蛋白和mRNA水平没有受到影响的阿司美坦在A549细胞，而增加的蛋白和mRNA水平观察到H23细胞，阿司美坦应用后48小时。此外，在两种细胞系中发现cAMP水平增加，15分钟后给予阿司美坦。此外，我们还研究了依西美坦对表皮生长因子受体（EGFR）定位和活化的影响。依西美坦在H23细胞中应用后15分钟增加EGFR活化。此外，我们证明在H23细胞中添加依西美坦24小时后，EGFR从细胞膜上移位。在A549细胞中未观察到EGFR活化或定位的变化。我们的研究结果表明，阿司美坦对NSCLC细胞系的抗增殖作用。依西美坦在芳香酶水平较高的细胞中可能更有效。需要进一步的研究来评估阿司美坦在NSCLC中的活性。
Recent evidence suggests that estrogen signaling may be involved in the pathogenesis of non-small cell lung cancer (NSCLC). Aromatase is an enzyme complex that catalyses the final step in estrogen synthesis and is present in several tissues, including the lung. In the current study we investigated the activity of the aromatase inhibitor exemestane in human NSCLC cell lines H23 and A549. Aromatase expression was detected in both cell lines. H23 cells showed lower protein and mRNA levels of aromatase, compared to A549 cells. Exemestane decreased cell proliferation and increased apoptosis in both cell lines, 48 h after its application, with A549 exhibiting higher sensitivity than H23 cells. Aromatase protein and mRNA levels were not affected by exemestane in A549 cells, whereas an increase in both protein and mRNA levels was observed in H23 cells, 48 h after exemestane application. Moreover, an increase in cAMP levels was found in both cell lines, 15 min after the administration of exemestane. In addition, we studied the effect of exemestane on epidermal growth factor receptor (EGFR) localization and activation. Exemestane increased EGFR activation 15 min after its application in H23 cells. Furthermore, we demonstrated a translocation of EGFR from cell membrane, 24 h after the addition of exemestane in H23 cells. No changes in EGFR activation or localization were observed in A549 cells. Our findings suggest an antiproliferative effect of exemestane on NSCLC cell lines. Exemestane may be more effective in cells with higher aromatase levels. Further studies are needed to assess the activity of exemestane in NSCLC.
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影响因子：
51.1
作者：
Dubey, Sarita;Siegfried, Jill M.;Traynor, Anne M.
通讯作者：
Traynor, Anne M.
影响因子：
21.3
作者：
Lin, SY;Makino, K;Hung, MC
通讯作者：
Hung, MC
影响因子：
11.2
作者：
Mah, Vei;Seligson, David B.;Goodglick, Lee
通讯作者：
Goodglick, Lee
影响因子：
--
作者：
Hong, Yanyan;Yu, Bin;Chen, Shiuan
通讯作者：
Chen, Shiuan
DOI：
10.1016/s0960-0760(01)00132-7
发表时间：
2001-12-01
影响因子：
4.1
作者：
Chen, SU;Zhou, DJ;Itoh, T
通讯作者：
Itoh, T