Identification of a novel human papilloma virus in cutaneous warts of meathandlers.
Identification of a novel human papilloma virus in cutaneous warts of meathandlers.
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肉类处理者皮肤疣中新型人乳头状瘤病毒的鉴定。
DOI：
10.1016/0042-6822(81)90524-9
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发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
Faras,AJ
中科院分区：
文献类型：
--
作者：
Ostrow,RS;Krzyzek,R;Pass,F;Faras,AJ
对来自四名肉类加工工人的常见皮肤疣的乳头瘤病毒 DNA 进行了表征，并将其与已知的人类皮肤乳头瘤病毒的 DNA 基因组进行了比较。从其中一份人乳头瘤样本中纯化病毒，发现其表现出与已知人乳头瘤病毒 (HPV) 不同的限制性核酸内切酶切割模式。此外，从其他三名患者的乳头状瘤中提取的病毒 DNA 表现出与这种新型病毒 DNA 相似的序列同源性和限制性内切核酸酶切割模式。除了这种独特的 HPV 之外，其中一名患者还含有第二种 HPV，它与 HPV-2 相关，但又不同，HPV-2 是一种与人类常见皮肤疣相关的 HPV。最后，采用 Southern 转移程序和严格的杂交条件，发现所研究的每个肉类加工商 HPV DNA 与主要的牛乳头瘤病毒 1 型和 2 型无关。
Papillomavirus DNA from common skin warts of four meathandlers has been characterized and compared to the DNA genomes of known human cutaneous papillomaviruses. Virus was purified from one of the human papilloma samples and was found to exhibit a restriction endonuclease cleavage pattern distinct from those of known human papillomaviruses (HPV). Moreover, viral DNA extracted from papillomas from the other three patients exhibited sequence homology and restriction endonuclease cleavage patterns similar to this novel viral DNA. In addition to this unique HPV, one of the patients also contained a second species of HPV which was related to, yet distinct from, HPV-2, a species of HPV associated with common cutaneous warts in man. Finally, employing the Southern transfer procedure and stringent hybridization conditions, each meathandler HPV DNA studied was found to be unrelated to the major species of bovine papillomavirus, types 1 and 2.