Sulforaphane and myricetin act synergistically to induce apoptosis in 3T3‑L1 adipocytes.
Sulforaphane and myricetin act synergistically to induce apoptosis in 3T3‑L1 adipocytes.
复制标题
磺胺素和迈雷丁在3T3 -L1脂肪细胞中诱导凋亡。
DOI：
10.3892/mmr.2017.8235
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发表时间：
2018-03
影响因子：
3.4
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Yao A;Shen Y;Zhang Z;Zou Z;Wang A;Chen S;Zhang H;Chen F;Zhao J;Chen Z;Shan Y;Zhang X
本研究的目的是探讨萝卜硫素（SFN）和杨梅素（Myr）是否协同诱导脂肪细胞凋亡。40µM SFN和/或100µM Myr处理的成熟3T3-L1脂肪细胞的活力采用MTT法评估。通过Hoechst 33258核染色和酶联免疫吸附法检测单链DNA来评估细胞凋亡。与单独使用相比，SFN和Myr联合使用可协同降低细胞活力，诱导凋亡，增加促凋亡的Bcl-2相关X蛋白表达，降低抗凋亡的b细胞淋巴瘤-2表达，增强Bcl-2相关死亡启动子（Bad）从细胞质向线粒体的易位，降低Bad在Ser112位点的磷酸化。这些影响伴随着半胱天冬酶3和聚adp核糖聚合酶的增加裂解。此外，SFN和Myr联合处理显著降低磷酸化AKT丝氨酸/苏氨酸激酶1 (AKT) Ser473位点的蛋白表达水平，以及下游蛋白核糖体蛋白S6激酶β-1的磷酸化水平。因此，SFN加Myr在3T3-L1脂肪细胞中比单独使用任何一种化合物更有效地诱导细胞凋亡。本研究结果提示，SNF/ myr诱导细胞凋亡的机制涉及激活akt介导的线粒体凋亡通路。这可能有助于肥胖动物模型的治疗和临床前试验。
The aim of the present study was to investigate whether sulforaphane (SFN) and myricetin (Myr) synergistically induce apoptosis in adipocytes. The viability of mature 3T3-L1 adipocytes treated with 40 µM SFN and/or 100 µM Myr was assessed using an MTT assay. Apoptosis was assessed by Hoechst 33258 nuclear staining, and by detection of single-stranded DNA using an enzyme-linked immunosorbent assay. Compared with the effects of each compound alone, the combination of SFN and Myr synergistically reduced cell viability, induced apoptosis, increased pro-apoptotic Bcl-2 associated X protein expression, decreased anti-apoptotic B-cell lymphoma-2 expression, enhanced Bcl-2-associated death promoter (Bad) translocation from the cytoplasm to the mitochondria, and reduced Bad phosphorylation at Ser112. These effects were accompanied by increased cleavage of caspase 3 and poly-ADP-ribose-polymerase. In addition, combined SFN and Myr treatment significantly decreased the protein expression levels of phosphorylated AKT serine/threonine kinase 1 (Akt) at Ser473, as well as the phosphorylation of the downstream protein ribosomal protein, S6 kinase β-1. Therefore, SFN plus Myr was a more potent inducer of apoptosis in 3T3-L1 adipocytes than either compound alone. The results of the present study suggest that the mechanism of SNF/Myr-induced apoptosis involved activation of the Akt-mediated mitochondrial apoptotic pathway. This may aid treatment of animal models of obesity and preclinical testing.
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DOI：
10.1158/1078-0432.ccr-09-2937
发表时间：
2010-05-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Li Y;Zhang T;Korkaya H;Liu S;Lee HF;Newman B;Yu Y;Clouthier SG;Schwartz SJ;Wicha MS;Sun D
通讯作者：
Sun D
DOI：
10.1016/j.bbrc.2012.09.006
发表时间：
2012-10-12
影响因子：
3.1
作者：
Sarkar, Ruma;Mukherjee, Sutapa;Roy, Madhumita
通讯作者：
Roy, Madhumita
影响因子：
9.7
作者：
Phillips, P. A.;Sangwan, V.;Borja-Cacho, D.;Dudeja, V.;Vickers, S. M.;Saluja, A. K.
通讯作者：
Saluja, A. K.
影响因子：
4.5
作者：
Lee, Yoon-Jin;Lee, Sang-Han
通讯作者：
Lee, Sang-Han
杨梅素在体外和体内对人 T24 膀胱癌细胞的潜在抗癌活性
DOI：
10.1080/01635581.2012.665564
发表时间：
2012-01-01
影响因子：
2.9
作者：
Sun, Fang;Zheng, Xiang Yi;Chen, Weilin
通讯作者：
Chen, Weilin