Cellular immune responses in peripheral blood lymphocytes of Giardia infected squirrel monkey (Saimiri boliviensis boliviensis) treated with Fenbendazole.
Cellular immune responses in peripheral blood lymphocytes of Giardia infected squirrel monkey (Saimiri boliviensis boliviensis) treated with Fenbendazole.
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DOI：
10.1371/journal.pone.0198497
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Vanchiere JA
中科院分区：
文献类型：
--
作者：
Nehete PN;Wilkerson G;Nehete BP;Chitta S;Ruiz JC;Scholtzova H;Williams LE;Abee CR;Vanchiere JA
Cellular immune responses were tested to determine the effect of fenbendazole on the function of lymphocytes from Bolivian squirrel monkeys (Samiri boliviensis boliviensis). Giardia-infected squirrel monkeys were treated with commercially available fenbendazole (FBZ)-medicated monkey chow. Immune responses were compared between historical controls (Giardia naïve, untreated with FBZ (control animals)) and Giardia-infected, FBZ-treated squirrel monkeys (study animals). Peripheral blood lymphocytes from study monkeys had significantly lower stimulation indices compared to control animals when cultured in vitro with concanavalin A (Con A) (p<0.0001), phytohaemagglutinin (PHA) (p<0.0001) and lipopolysaccharide (LPS) (p<0.0001). PBMCs were also analyzed for IFN-γ producing cells in response to stimulation with Con A, PHA, PWM, and LPS by the cytokine ELISPOT assay. Significantly higher responses to Con A- (p<0.0001), and PHA- (p<0.001) stimulated cultures from Giardia-infected and fenbendazole treated compared to controls. Flow cytometric analysis for expression of cell surface markers revealed a significant increase in B- and NKT-lymphocytes and significant decrease in CD14+CD16+ monocytes after FBZ treatment. Also, circulating plasma cytokines IFN-γ, TNF-α, IL-12p40, IL-1β, IL-10, IL-13, IL-1ra, IL-6 and IL-4 were significantly decreased after FBZ treatment. Comparison of hematologic parameters between controls and FBZ-treated squirrel monkeys revealed significantly lower numbers of total leukocytes, neutrophils, monocytes, and eosinophils compared to controls. However, erythrocyte indices (red cell count, hemoglobin and hematocrit were significantly higher in FBZ-treated monkeys. Our findings suggest that fenbendazole treatment may alter sensitive immune and molecular measures of inflammation. Postponing the experimental use of squirrel monkeys until at least 6 weeks after FBZ treatment should be considered.
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影响因子：
1.4
作者：
CABAJ, W;STANKIEWICZ, M;MOORE, LG
通讯作者：
MOORE, LG
影响因子：
5.5
作者：
Nehete, PN;Nehete, BP;Sastry, KJ
通讯作者：
Sastry, KJ
DOI：
10.1023/a:1008099612354
发表时间：
1998-01-01
期刊：
BIOTHERAPY
影响因子：
--
作者：
Holcombe, RF;Li, AL;Stewart, RM
通讯作者：
Stewart, RM
影响因子：
2.2
作者：
Grit, G. H.;Devriendt, B.;Claerebout, E.
通讯作者：
Claerebout, E.
DOI：
10.1002/em.2860020110
发表时间：
1980-01-01
期刊：
ENVIRONMENTAL MUTAGENESIS
影响因子：
--
作者：
HOLDEN, HE;CRIDER, PA;WAHRENBURG, MG
通讯作者：
WAHRENBURG, MG