Comparison of toxicokinetics and toxic effects of PFOS and its novel alternative OBS in zebrafish larvae
Comparison of toxicokinetics and toxic effects of PFOS and its novel alternative OBS in zebrafish larvae
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PFOS 及其新型替代 OBS 对斑马鱼幼虫的毒代动力学和毒性作用比较
DOI：
10.1016/j.chemosphere.2020.129116
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发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
Wenqing Tu
中科院分区：
文献类型：
--
作者：
Yilong Zou;Yongming Wu;Qiyu Wang;Jinbao Wan;Mi Deng;Wenqing Tu
全氟壬烯氧基苯磺酸钠（OBS）是全氟辛烷磺酸盐（PFOS）的新型替代品，作为表面活性剂、消防泡沫和感光材料广泛应用于工业中。最近有关于OBS在水生环境中出现的报道，但关于其在水生生物中的积累和毒性影响的信息很少。在这项研究中，斑马鱼幼鱼（受精后3天）接受了为期48小时的OBS（10、100微克/升）和全氟辛烷磺酸（10微克/升）处理，随后进行了24小时的净化。研究了OBS和PFOS在斑马鱼幼鱼体内的生物富集和净化动力学、氧化应激及其可能的分子机制。结果表明，水体对OBS和PFOS的吸收和净化均符合一级动力学模型。OBS的吸收速率常数与全氟辛烷磺酸相似，但净化速率常数远高于全氟辛烷磺酸，其半衰期分别为69.7-85 h和222.2 h。计算得出的OBS和全氟辛烷磺酸的生物浓缩系数分别为238.0-242.5和644.2。在急性毒性实验中，Nrf 2蛋白表达增强，CAT和SOD蛋白表达上调，表明OBS和PFOS诱导了斑马鱼幼鱼的氧化应激，Nrf 2-ARE信号通路参与了这一过程。总的来说，OBS的生物浓缩潜力低于全氟辛烷磺酸，但其对斑马鱼幼体氧化应激的毒性效应与全氟辛烷磺酸相当。
Sodiump-perfluorous nonenoxybenzene sulfonate (OBS), a novel alternative to perfluorooctanesulfonate (PFOS), is widely used in industry as a surfactant, firefighting foam and photographic material. The occurrence of OBS in the aquatic environment has been recently reported, but little information is available on its accumulation and toxic effects in aquatic organisms. In this study, zebrafish larvae (3 d post-fertilization) were subjected to OBS (10, 100 μg/L) and PFOS (10 μg/L) for a period of 48 h, followed by a 24 h of depuration period. The bioconcentration and depuration kinetics, oxidative stress and possible molecular mechanisms of OBS and PFOS were investigated in zebrafish larvae. Our results showed that the uptake and depuration of both OBS and PFOS fitted well with a first-order kinetic model. The uptake rate constant of OBS was similar to that of PFOS, but the depuration rate constant was much higher than PFOS with a half-life of 69.7–85 h for OBS and 222.2 h for PFOS. The calculated BCFs of OBS and PFOS were 238.0–242.5 and 644.2, respectively. In our acute toxicity assay, the enhanced expression of Nrf2 protein accompanied by the upregulation of CAT and SOD protein expressions indicated OBS and PFOS induced oxidative stress in zebrafish larvae, and the Nrf2-ARE signaling pathway was involved in this process. Collectively, OBS has a lower bioconcentration potential than PFOS, but its toxic effect on oxidative stress was comparable to PFOS in zebrafish larvae.