Expression of the neutrophil elastase gene during human bone marrow cell differentiation.
Expression of the neutrophil elastase gene during human bone marrow cell differentiation.
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DOI：
10.1084/jem.169.3.833
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发表时间：
1989-03-01
期刊：
影响因子：
--
通讯作者：
Crystal RG
中科院分区：
文献类型：
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作者：
Fouret P;du Bois RM;Bernaudin JF;Takahashi H;Ferrans VJ;Crystal RG
中性粒细胞弹性蛋白酶是由活化的中性粒细胞携带和释放的一种有效的丝氨酸蛋白酶，不是由中性粒细胞合成，而是由其骨髓前体细胞合成。使用原位杂交与35 S标记的反义和正义中性粒细胞弹性蛋白酶cRNA探针，本研究表明，中性粒细胞弹性蛋白酶基因的表达是严格控制在骨髓前体细胞和中性粒细胞髓系的分化过程中发生在一个非常有限的阶段，几乎完全在早幼粒细胞阶段。神经弹性蛋白酶mRNA转录水平在原始细胞中可检测到有限程度，在早幼粒细胞阶段显著增加，然后随着早幼粒细胞进一步分化而消失。分别对髓过氧化物酶、乳铁蛋白和β-珠蛋白mRNA转录物具有特异性的对照探针显示出对比的基因表达。髓过氧化物酶mRNA转录也发现几乎完全在早幼粒细胞阶段，但髓过氧化物酶mRNA水平消失早于中性粒细胞弹性蛋白酶mRNA水平，这表明这些基因的表达可能是不同的控制。相比之下，乳铁蛋白mRNA转录物在嗜中性粒细胞谱系中检测到较晚，而β-珠蛋白mRNA仅在红系谱系的细胞中检测到。总之，这些观察结果表明，中性粒细胞弹性蛋白酶基因的表达可能是在非常严格的控制下，并可能不同于其他成分的中性粒细胞颗粒。
Neutrophil elastase, a potent serine protease carried and released by activated neutrophils, is not synthesized by neutrophils, but by their bone marrow precursor cells. Using in situ hybridization with 35S- labeled antisense and sense neutrophil elastase cRNA probes, the present study demonstrates that expression of the neutrophil elastase gene is tightly controlled in bone marrow precursors and occurs during a very limited stage of differentiation of the neutrophil myeloid series, almost entirely at the promyelocyte stage. Neutrophil elastase mRNA transcript levels are detectable to a limited extent in blasts, increase markedly in the promyelocyte stage, and then disappear as promyelocytes further differentiate. Control probes specific for myeloperoxidase, lactoferrin, and beta-globin mRNA transcripts, respectively, demonstrated contrasting gene expression. Myeloperoxidase mRNA transcripts were also found almost exclusively at the promyelocyte stage, but myeloperoxidase mRNA levels disappeared earlier than do neutrophil elastase mRNA levels, suggesting that expression of these genes may be differently controlled. In comparison, lactoferrin mRNA transcripts were detected late in the neutrophil lineage, while beta- globin mRNA was detected only in cells of the erythroid lineage. Together these observations suggest that the expression of the neutrophil elastase gene is likely under very tight control, and is likely different than that for other constituents of the neutrophil granules.