Increasing water-use efficiency directly through genetic manipulation of stomatal density
Increasing water-use efficiency directly through genetic manipulation of stomatal density
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DOI：
10.1111/nph.13347
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发表时间：
2015-07-01
期刊：
影响因子：
9.4
通讯作者：
Gray, Julie E.
中科院分区：
文献类型：
--
作者：
Franks, Peter J.;Doheny-Adams, Timothy W.;Gray, Julie E.
提高作物水分利用效率（WUE）是面临干旱加剧或地下水资源减少的地区的一个关键优先事项。尽管有新的工具来操纵气孔发育，但高WUE植物的工程化仍然是一个挑战。我们使用拟南芥表皮图案形成因子（EPF）突变体表现出改变气孔密度来测试是否可以直接通过操纵控制气孔密度的基因来提高WUE。具体而言，我们测试是否组成性过度表达EPF2减少气孔密度和最大气孔导度（g（w（max）足以增加WUE。我们发现，减少g（w（最大））通过减少气孔密度在EPF2过表达植物（EPF2OE）增加瞬时和长期的WUE没有改变显着的光合能力。相反，植物缺乏EPF1和EPF2的表达（epf1epf2）表现出较高的气孔密度，较高的g（w（max））和较低的瞬时WUE，以及较低（但不显着）的长期WUE。气孔导度的靶向遗传修饰，如EPF2OE，是一种可行的方法，在作物中，特别是在未来的高二氧化碳（CO2）的气氛中更高的WUE的工程。
Improvement in crop water-use efficiency (WUE) is a critical priority for regions facing increased drought or diminished groundwater resources. Despite new tools for the manipulation of stomatal development, the engineering of plants with high WUE remains a challenge. We used Arabidopsis epidermal patterning factor (EPF) mutants exhibiting altered stomatal density to test whether WUE could be improved directly by manipulation of the genes controlling stomatal density. Specifically, we tested whether constitutive overexpression of EPF2 reduced stomatal density and maximum stomatal conductance (g(w(max))) sufficiently to increase WUE. We found that a reduction in g(w(max)) via reduced stomatal density in EPF2-overexpressing plants (EPF2OE) increased both instantaneous and long-term WUE without altering significantly the photosynthetic capacity. Conversely, plants lacking both EPF1 and EPF2 expression (epf1epf2) exhibited higher stomatal density, higher g(w(max)) and lower instantaneous WUE, as well as lower (but not significantly so) long-term WUE. Targeted genetic modification of stomatal conductance, such as in EPF2OE, is a viable approach for the engineering of higher WUE in crops, particularly in future high-carbon-dioxide (CO2) atmospheres.