Characterization of HIV-1 integrase N-terminal mutant viruses.
Characterization of HIV-1 integrase N-terminal mutant viruses.
复制标题
HIV-1 整合酶 N 末端突变病毒的表征。
DOI：
10.1016/j.virol.2006.10.007
复制
发表时间：
2007
期刊：
影响因子：
3.7
通讯作者：
Mulder,LubbertusCF
中科院分区：
文献类型：
--
作者：
Lloyd,AlizaG;Ng,YenShing;Muesing,MarkA;Simon,Viviana;Mulder,LubbertusCF
在感染过程中，人类免疫缺陷病毒1型整合酶参与许多病毒和细胞来源的分子和机制。在其中一种情况下，整合酶被认为是通过N-末端规则蛋白酶体途径降解的，这是一种靶向其底物的N-末端残基的过程。在这里，我们描述了HIV-1病毒的特性，其中整合酶的第一个氨基酸残基已被取代，使其耐N-末端规则途径。由于这种交换，我们观察到一组I类和II类缺陷，导致病毒复制效率大幅降低。具体而言，逆转录和整合是似乎受到影响的步骤。我们建议，这些突变体的严重缺陷施加强大的选择压力，导致在HIV-1，HIV-2和SIV的整合酶的N-末端残基的几乎完全保守。
During infection, human immunodeficiency virus type 1 integrase engages a number of molecules and mechanisms, both of viral and cellular origin. In one of such instances, integrase is thought to be degraded by the N-end rule proteasome pathway a process that targets the N-terminal residue of its substrates. Here we describe the properties of HIV-1 viruses in which the first amino acid residue of integrase has been substituted to render it resistant to the N-end rule pathway. As result of this exchange, we observe a set of class I and class II defects that result in a large decrease of viral replication efficiency. Specifically, reverse transcription and integration are the steps that appear to be affected. We propose that the severe deficiency of these mutants exert a strong selective pressure that leads to the near total conservation of the N-terminal residue of integrase in HIV-1, HIV-2 and SIV.