Feeding of pregnant sows with mycotoxin-contaminated diets and their non-effect on foetal and maternal hepatic transcription of genes of the insulin-like growth factor system
Feeding of pregnant sows with mycotoxin-contaminated diets and their non-effect on foetal and maternal hepatic transcription of genes of the insulin-like growth factor system
复制标题
DOI：
10.1080/02652030802112619
复制
发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
U. Tiemann;K. Brüssow;Sven Dänicke;Jens Vanselow
中科院分区：
文献类型：
--
作者：
U. Tiemann;K. Brüssow;Sven Dänicke;Jens Vanselow
胰岛素样生长因子（IGF）系统调节胎儿和胎盘的生长。它可以受到饮食因素的影响，但很少有人知道霉菌毒素喂养对IGF系统的肝脏水平的影响。目的是（1）确定在妊娠的不同阶段主要IGF基因的正常胎儿和母体肝脏转录，以及（2）找出真菌毒素脱氧雪腐镰刀菌烯醇（DON）和玉米赤霉烯酮（ZON）是否影响转录物浓度。妊娠母猪从第35天至第70天（实验1），或从第75天至第110天（实验2），或与对照饮食喂养自然污染的饮食。在第70天和第110天通过定量聚合酶链反应（qPCR）测定胎肝IGF转录物，在第70天测定母体转录物。在母猪中发现IGF-I转录水平最高。相反，IGF-II主要在第70天的胎儿中表达。IGF-IR和IGFBP-3的表达逐渐下降，而IGFBP-2的表达逐渐增加。IGF-IIR和IGFBP-1表达在胎儿和足月仔猪中相似。IGF-IIR显示母体肝脏中的水平降低。妊娠母猪暴露于DON和ZON污染的饮食显着降低了母体，并倾向于降低实验2中仔猪的体重增加，但在两个实验中对肝脏IGF转录水平没有影响。这表明，霉菌毒素污染的饮食可以诱导猪在怀孕期间的生长抑制，而不影响主要IGF基因的肝脏转录。
The insulin-like growth factor (IGF) system regulates foetal and placental growth. It can be influenced by dietary factors, but little is known about the effects of mycotoxin feeding on hepatic levels of the IGF system. The aim was (1) to determine the normal foetal and maternal hepatic transcription of major IGF genes at distinct stages of pregnancy and (2) to find out if the mycotoxins deoxynivalenol (DON) and zearalenone (ZON) affect transcript concentrations. Pregnant sows were fed with naturally contaminated diets from days 35 to 70 (experiment 1), or from days 75 to 110 (experiment 2), or with control diets. Foetal hepatic IGF transcripts were determined at days 70 and 110, maternal transcripts at day 70 by quantitative polymerase chain reaction (qPCR). The highest levels of IGF-I transcripts were found in sows. On the contrary, IGF-II was predominantly expressed in foetuses at day 70. Expression of IGF-IR and IGFBP-3 decreased, whereas that of IGFBP-2 increased towards term. IGF-IIR and IGFBP-1 expression was similar in both foetuses and full-term piglets. IGF-IIR showed reduced levels in the maternal liver. The exposure of pregnant sows to DON- and ZON-contaminated diets significantly reduced the maternal and tended to reduce the weight gain of piglets in experiment 2, but had no effects on hepatic levels of IGF transcripts in both experiments. This suggests that mycotoxin-contaminated diets can induce growth depression in pigs during pregnancy without affecting hepatic transcription of major IGF genes.