Construction and use of glycan microarrays.
Construction and use of glycan microarrays.
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DOI：
10.1002/9780470559277.ch090228
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发表时间：
2010-03-01
影响因子：
--
通讯作者：
Gildersleeve JC
中科院分区：
文献类型：
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作者：
Campbell CT;Zhang Y;Gildersleeve JC
糖基化是一种重要的翻译后修饰，影响许多对发育、正常生理功能和疾病至关重要的生物过程。不幸的是，由于研究聚糖及其相互作用的蛋白质所面临的挑战，了解聚糖在生物学中的作用的进展一直缓慢。聚糖微阵列为糖-大分子相互作用的快速分析提供了一种高通量的方法。这里详细的协议旨在帮助实验室与DNA或蛋白质微阵列的基本熟悉，开始打印和执行检测使用聚糖微阵列。还详细介绍了基本和高级数据处理，以及提高聚糖阵列收集数据的可重复性的策略。
Glycosylation is an important post-translational modification that influences many biological processes critical for development, normal physiologic function, and diseases. Unfortunately, progress towards understanding the roles of glycans in biology has been slow due to the challenges of studying glycans and the proteins that interact with them. Glycan microarrays provide a high-throughput approach for the rapid analysis of carbohydrate-macromolecule interactions. Protocols detailed here are intended to help laboratories with basic familiarity of DNA or protein microarrays to begin printing and performing assays using glycan microarrays. Basic and advanced data processing are also detailed, along with strategies for improving reproducibility of data collected with glycan arrays.