High performance DNA nano-carriers of carbonate apatite: Multiple factors in regulation of particle synthesis and transfection efficiency
High performance DNA nano-carriers of carbonate apatite: Multiple factors in regulation of particle synthesis and transfection efficiency
复制标题
DOI：
10.2147/nano.2007.2.1.101
复制
发表时间：
2007-01-01
影响因子：
8
通讯作者：
Aklike, Toshihiro
中科院分区：
文献类型：
--
作者：
Chowdhury, E. H.;Aklike, Toshihiro
考虑到病毒颗粒的一些潜在的威胁生命的作用，越来越多的关注合成DNA递送系统，以发展基于基因的纳米药物在21世纪世纪。在目前的非病毒方法中，大部分的努力都是与有机大分子如脂质，聚合物和肽，但相对较少的尝试，以评估无机材料的基因传递的潜力。我们最近报道了可生物降解的碳酸磷灰石纳米颗粒在转染多种哺乳动物细胞方面非常有效。在这里，我们表明，一些参数积极调节纳米颗粒的合成及其随后的转染效率。发展的“过饱和”，这是产生这样的颗粒的先决条件，可以很容易地调节反应物浓度，缓冲溶液的pH值，和孵育温度，使我们能够建立一个灵活的颗粒生成过程，高生产力的转基因传递。已经提出碳酸盐掺入颗粒中用于产生纳米尺寸的颗粒，导致细胞摄取大量质粒DNA以及内体去稳定化，从而促进DNA从内体的显著释放。
Increasing attention is being paid on synthetic DNA delivery systems considering some potential life-threatening effects of viral particles, for development of gene-based nano-medicine in the 21st century. In the current nonviral approaches, most of the efforts have been engaged with organic macromolecules like lipids, polymers, and peptides, but comparatively fewer attempts were made to evaluate the potential of inorganic materials for gene delivery. We recently reported that biodegradable nanoparticles of carbonate apatite are highly efficient in transfecting a wide variety of mammalian cells. Here we show that a number of parameters actively regulate synthesis of the nanoparticles and their subsequent transfection efficacy. Development of "supersaturation", which is the prerequisite for generation of such particles, could be easily modulated by reactant concentrations, pH of the buffered solution, and incubation temperatures, enabling us to establish a flexible particle generation process for highly productive trans-gene delivery. Carbonate incorporation into the particles have been proposed for generating nano-size particles resulting in cellular uptake of huge amount of plasmid DNA as well as endosome destabilization facilitating significant release of DNA from the endosomes.