Stacked Thiazole Orange Dyes in DNA Capable of Switching Emissive Behavior in Response to Structural Transitions
Stacked Thiazole Orange Dyes in DNA Capable of Switching Emissive Behavior in Response to Structural Transitions
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DOI：
10.1002/cbic.202100309
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发表时间：
2021-06
期刊：
影响因子：
3.2
通讯作者：
T. Takada;K. Nishida;Yurika Honda;Aoi Nakano;Mitsunobu Nakamura;Shuya Fan;K. Kawai;M. Fujitsuka;K. Yamana
中科院分区：
文献类型：
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作者：
T. Takada;K. Nishida;Yurika Honda;Aoi Nakano;Mitsunobu Nakamura;Shuya Fan;K. Kawai;M. Fujitsuka;K. Yamana
具有响应杂交事件、微环境或结构变化而产生荧光的能力的功能核酸作为结构探针和化学传感器是有价值的。我们现在展示了具有多种噻唑橙子（TO）染料的核酸的酶辅助制备及其荧光行为，其显示出从典型单体发射到准分子型红移发射的光谱变化。我们发现TO染料的荧光响应和发射波长取决于DNA结构的状态（单链或双链DNA）和TO染料的排列。我们发现TO染料的荧光行为可以应用于RNA分子的检测，这表明我们制备用多个TO染料功能化的荧光核酸的方法可以用于设计生物分子的荧光生物成像和检测技术。
Functional nucleic acids with the capability of generating fluorescence in response to hybridization events, microenvironment or structural changes are valuable as structural probes and chemical sensors. We now demonstrate the enzyme‐assisted preparation of nucleic acids possessing multiple thiazole orange (TO) dyes and their fluorescent behavior, that show a spectral change from the typical monomer emission to the excimer‐type red‐shifted emission. We found that the fluorescent response and emission wavelength of the TO dyes were dependent on both the state of the DNA structure (single‐ or double‐stranded DNA) and the arrangement of the TO dyes. We showed that the fluorescent behavior of the TO dyes can be applied for the detection of RNA molecules, suggesting that our approach for preparing the fluorescent nucleic acids functionalized with multiple TO dyes could be useful to design a fluorescence bioimaging and detection technique of biomolecules.