Hydroxyapatite surface solubility and effect on cell adhesion
Hydroxyapatite surface solubility and effect on cell adhesion
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DOI：
10.1016/j.colsurfb.2010.02.027
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发表时间：
2010-07-01
影响因子：
5.8
通讯作者：
Bertran, Celso A.
中科院分区：
文献类型：
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作者：
Bertazzo, Sergio;Zambuzzi, Willian F.;Bertran, Celso A.
在生物体中，生物羟基磷灰石与体液（例如血清和唾液）持续接触。因此，溶解、溶解和沉淀是该材料与组织中生物液体相互作用的一部分。在这项工作中，我们已经获得了由水溶液形成的系统与羟基磷灰石平衡的溶解度常数，从而间接地获得了改性羟基磷灰石表面的组成。为了检查这种平衡的效果和羟基磷灰石表面在水溶液中遭受的改性，我们在平衡之前和之后在羟基磷灰石盘上培养前成骨细胞。结果揭示了羟基磷灰石生物活性机理的关键步骤，即羟基磷灰石的增溶和在表面形成平衡。这些过程修改羟基磷灰石表面，其组成改变为具有化学式CaHPO4的新的磷酸钙化合物。对羟基磷灰石表面发生的转化以及这些转化与细胞活性之间的相互作用的清楚描述是羟基磷灰石参与的过程的两个基本方面，例如骨替代、骨重建、骨质疏松症和龋齿。(C)2010爱思唯尔有限公司版权所有。
In living organisms the biological hydroxyapatite is in constant contact with body fluids, such as blood serum and saliva. Thus, dissolution, solubility and precipitation take place as part of the interaction of this material with biological fluids in tissues. In this work we have obtained the solubility constant for the system formed from aqueous solutions in equilibrium with hydroxyapatite and thus indirectly obtained the composition of the modified hydroxyapatite surface. In order to check the effects of this equilibrium and of the modification that the surface of hydroxyapatite suffers in aqueous solutions, we cultured pre-osteoblasts onto hydroxyapatite discs before and after equilibrium. The results revealed key steps of the mechanism for the bioactivity of hydroxyapatite, which are the solubilization of hydroxyapatite and the equilibrium that is formed on the surface. These processes modify the hydroxyapatite surface, whose composition is changed to a new calcium phosphate compound with the chemical formula of CaHPO4. A clear description of the transformations that occur on the surface of hydroxyapatite and of the interplay between these transformations and cell activity are two fundamental aspects of processes in which hydroxyapatite takes part, such as bone substitution, bone remodeling, osteoporosis and caries. (C) 2010 Elsevier B.V. All rights reserved.