The Urobiomes of Adult Women With Various Lower Urinary Tract Symptoms Status Differ: A Re-Analysis.
The Urobiomes of Adult Women With Various Lower Urinary Tract Symptoms Status Differ: A Re-Analysis.
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DOI：
10.3389/fcimb.2022.860408
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发表时间：
2022
影响因子：
5.7
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泌尿微生物组（urobiome）的发现为研究各种人类健康状况的泌尿健康研究人员创造了机会。本文描述了对从1，004名尿生物群系研究参与者中获得的导管尿液样本的分析，目的是在5个临床相关队列中确定最丰富和/或最普遍（常见）的分类群：未受影响的成年女性（n=346，34.6%），急迫性尿失禁（UUI）（n=255，25.5%）、压力性尿失禁（SUI）（n=50，5.0%）、尿路感染（UTI）（n=304，30.4%）和间质性膀胱炎/膀胱疼痛综合征（IC/PBS）（n=49，4.9%）。通过经尿道导管收集尿液，并使用扩展定量尿液培养（EQUC）技术评估微生物。对于该合并分析队列，平均年龄为59 ± 16岁;大多数为白人（n=704，70.2%）、黑人（n=137，13.7%）或西班牙裔（n=130，13.0%），平均BMI为30.4 ± 7.7。尽管许多对照组或IC/PBS组成员为EQUC阴性（分别为42.4%和39.8%），但其他3个组的成员极有可能具有可检测到的微生物。对照组和IC/PBS的检测到的尿生物组在α多样性或属水平组成方面没有差异，并且仅在几个物种方面存在差异。其他3个队列与对照组差异显著。正如预期的那样，埃希氏菌在UTI队列中既普遍又高度丰富，但其他分类群也以更中等的丰度普遍存在，包括乳杆菌属、链球菌属、葡萄球菌属、棒状杆菌属、放线菌属和气球菌属的成员。这些属的成员在UUI队列的成员中也普遍存在且高度丰富，尤其是咽峡炎链球菌。有趣的是，这些分类群也在对照组中检测到，但患病率和丰度都大大降低，这表明UUI相关症状可能是典型的尿生物组成分过多的结果。最后，SUI队列中微生物的患病率和丰度介于UUI和对照队列之间。这些观察结果可以为下一个十年的urobiome研究提供信息，目的是阐明urobiome群落组成和功能的机制。有巨大的潜力，以改善诊断，评估和治疗的个人受到各种各样的尿路疾病。
The discovery of the urinary microbiome (urobiome) has created opportunities for urinary health researchers who study a wide variety of human health conditions. This manuscript describes an analysis of catheterized urine samples obtained from 1,004 urobiome study participants with the goal of identifying the most abundant and/or prevalent (common) taxa in five clinically relevant cohorts: unaffected adult women (n=346, 34.6%), urgency urinary incontinence (UUI) (n=255, 25.5%), stress urinary incontinence (SUI) (n=50, 5.0%), urinary tract infection (UTI) (n=304, 30.4%), and interstitial cystitis/painful bladder syndrome (IC/PBS) (n=49, 4.9%). Urine was collected via transurethral catheter and assessed for microbes with the Expanded Quantitative Urine Culture (EQUC) technique. For this combined analytic cohort, the mean age was 59 ± 16; most were Caucasian (n=704, 70.2%), Black (n=137, 13.7%), or Hispanic (n=130, 13.0%), and the mean BMI was 30.4 ± 7.7. Whereas many control or IC/PBS cohort members were EQUC-negative (42.4% and 39.8%, respectively), members of the other 3 cohorts were extremely likely to have detectable microbes. The detected urobiomes of the controls and IC/PBS did not differ by alpha diversity or genus level composition and differed by only a few species. The other 3 cohorts differed significantly from the controls. As expected, Escherichia was both prevalent and highly abundant in the UTI cohort, but other taxa also were prevalent at more moderate abundances, including members of the genera Lactobacillus, Streptococcus, Staphylococcus, Corynebacterium, Actinomyces, and Aerococcus. Members of these genera were also prevalent and highly abundant in members of the UUI cohort, especially Streptococcus anginosus. Intriguingly, these taxa were also detected in controls but at vastly lower levels of both prevalence and abundance, suggesting the possibility that UUI-associated symptoms could be the result of an overabundance of typical urobiome constituents. Finally, prevalence and abundance of microbes in the SUI cohort were intermediate to those of the UUI and control cohorts. These observations can inform the next decade of urobiome research, with the goal of clarifying the mechanisms of urobiome community composition and function. There is tremendous potential to improve diagnosis, evaluation and treatment for individuals affected with a wide variety of urinary tract disorders.