Effects of elevated oxygen levels on VOC analysis by means of PTR-ToF-MS
Effects of elevated oxygen levels on VOC analysis by means of PTR-ToF-MS
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氧气含量升高对 PTR-ToF-MS VOC 分析的影响
DOI：
10.1088/1752-7163/ab28ec
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发表时间：
2019
影响因子：
3.8
通讯作者：
Miekisch
中科院分区：
文献类型：
--
作者：
Pugliese;Schubert;Miekisch
质子转移反应飞行时间质谱（PTR-ToF-MS）是一种实时监测人体呼吸中挥发性有机化合物（VOC）分布的有力工具。然而，样品基质中不同的氧气浓度可能会影响PTR-ToF-MS的VOC分析结果。氧气浓度升高可能发生在临床环境中，也可能发生在使用呼吸面罩或呼吸装置时（例如，水肺潜水，航空，消防）。受试者之间或测量或研究过程中的氧浓度可能会有所不同，因此会导致结果偏倚。我们系统地评估了样品基质中高O2浓度（高达90%）对干燥和潮湿样品中包括芳香族化合物，醛和酮在内的VOC模式的PTR-MS分析结果的影响。在健康志愿者和机械通气动物中进行了体内实验，以测试在现实生活条件下的效果。H3 O+计数显着下降超过40%，当样品基质中的氧气量增加。几乎所有的研究挥发性有机化合物显着影响不同的氧气浓度和信号强度的差异超过50%，可以观察到。这种效应在干燥的样品中通常更明显，但在潮湿的条件下仍然很明显。观察到一些VOC（如醛类）的灵敏度对样品基质中氧浓度的线性依赖性，可能使基于因子的校正成为可能。在体内条件下，VOC强度也受到影响，例如乙醇降低高达71%。当在供氧条件下进行PTR-MS分析时，需要仔细考虑这些问题。
Proton-transfer-reaction-time-of-flight-mass-spectrometry (PTR-ToF-MS) is a powerful tool for real-time monitoring of volatile organic compound (VOC) profiles in human breath. However, varying oxygen concentrations in the sample matrix may influence results from VOC analysis by PTR-ToF-MS. Elevated oxygen concentrations are likely to occur in clinical settings, but also when respiratory masks or breathing apparatus are used (eg in scuba diving, aviation, firefighting). Oxygen concentration may vary between subjects or within the course of a measurement or study and thus bias results. We systematically assessed the effect of high O 2 concentrations (up to 90%) in the sample matrix on the results of PTR-MS analysis for a pattern of VOCs including aromatics, aldehydes and ketones in dry and humid samples. In vivo experiments in healthy volunteers and mechanically ventilated animals were done to test the effect under real-life conditions. H 3 O+ count significantly decreased by more than 40% when the amount of oxygen in the sample matrix was increased. Almost all investigated VOCs were significantly effected by varying oxygen concentrations and differences in signal intensities of more than 50% could be observed. The effect was generally more pronounced in dry samples but still significant under humid conditions. A linear dependency of sensitivity on the oxygen concentration in the sample matrix was observed for a number of VOCs (eg aldehydes) possibly enabling a factor based correction. VOC intensities were also influenced under in vivo conditions, eg ethanol decreased up to 71%. When PTR-MS analysis is carried out under oxygen supply, these issues need to be carefully considered.
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影响因子：
7.4
作者：
Blake, RS;Whyte, C;Monks, PS
通讯作者：
Monks, PS
影响因子：
1.8
作者：
Blake, Robert S.;Wyche, Kevin P.;Monks, Paul S.
通讯作者：
Monks, Paul S.
DOI：
--
发表时间：
1968
期刊：
影响因子：
--
作者：
V. H. Dibeler;Susan K. Liston
通讯作者：
Susan K. Liston
影响因子：
3.8
作者：
Sukul, Pritam;Trefz, Phillip;Miekisch, Wolfram
通讯作者：
Miekisch, Wolfram
提高质子转移反应质谱仪的检测限：在线监测几个pptv混合比例的挥发性有机化合物
DOI：
--
发表时间：
1998
期刊：
影响因子：
--
作者：
A. Hansel;A. Jordan;C. Warneke;R. Holzinger;W. Lindinger
通讯作者：
W. Lindinger