Isolation and expression analysis of 18 CsbZIP genes implicated in abiotic stress responses in the tea plant (Camellia sinensis)
Isolation and expression analysis of 18 CsbZIP genes implicated in abiotic stress responses in the tea plant (Camellia sinensis)
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DOI：
10.1016/j.plaphy.2015.10.030
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发表时间：
2015-12-01
影响因子：
6.5
通讯作者：
Yang, Yajun
中科院分区：
文献类型：
--
作者：
Cao, Hongli;Wang, Lu;Yang, Yajun
碱性亮氨酸拉链（bZIP）转录因子（TF）在植物逆境胁迫过程中起着重要的调控作用。尽管这种木本作物的经济重要性，有很少的信息bZIP TF在茶树。在这项研究中，18 bZIP基因分离的茶树（茶树），并命名为CsbZIP 1到CsbZIP 18。根据拟南芥的系统发育分类，CsbZIP基因跨越了bZIP转录因子的10个亚组（A、B、C、D、E、F、H、I、S和K组）。当分析器官特异性表达时，发现所有CsbZIP基因在根、茎、叶和花中普遍表达。CsbZIP基因在四种非生物胁迫下的表达分析表明，在叶片中，9、9、15和11个CsbZIP基因在低温、外源阿坝、高盐和脱水条件下的转录本丰度分别有2倍以上的变化。在根中，5，12，14和11 CsbZIPs的差异表达条件下的寒冷，外源阿坝，高盐和脱水胁迫。F、H、S和K组的CsbZIP基因对上述4种胁迫均表现出数倍的上调和/或下调。值得注意的是，K组的CsbZIP 18在响应这些相同的应激时表现出显着上调，这表明在应激反应中发挥着重要的功能作用。总之，这些发现增加了我们对茶树中bZIP TF的了解，并表明CsbZIP基因在植物非生物反应中的重要性。(C)2015年Elsevier Masson SAS。All rights reserved.
Basic leucine zipper (bZIP) transcription factors (TFs) play essential roles in regulating stress processes in plants. Despite the economic importance of this woody crop, there is little information about bZIP TFs in tea plants. In this study, 18 bZIP genes were isolated from the tea plant (Camellia sinensis) and named sequentially from CsbZIP1 to CsbZIP18. According to the phylogenetic classification as in Arabidopsis, the CsbZIP genes spanned ten subgroups (Group A, B, C, D, E, F, H, I, S and K) of bZIP TFs. When analyzed for organ specific expression, all CsbZIP genes were found to be ubiquitously expressed in roots, stems, leaves and flowers. Expression analysis of CsbZIP genes in response to four abiotic stresses showed that in leaves, 9, 9, 15 and 11 CsbZIPs have 2-fold greater variation in transcript abundance under cold, exogenous ABA, high salinity and dehydration conditions, respectively. In roots, 5, 12, 14 and 11 CsbZIPs were differentially expressed under conditions of cold, exogenous ABA, high salinity and dehydration stresses. Moreover, CsbZIP genes in Groups F, H, S and K exhibited several folds up-and/or down-regulation against the above four stresses. Notably, CsbZIP18 of group K showed significant up-regulation in response to these same stresses, suggesting a vital functional role in stress response. Together, these findings increase our knowledge of bZIP TFs in the tea plant and suggest the significance of CsbZIP genes in plant abiotic responses. (C) 2015 Elsevier Masson SAS. All rights reserved.