BcMF5, a pollen coat protein gene (PCP), from Brassica rapa. ssp. chinensis, involved in the transcription of different lengths of 3′-UTRs of PCPs
BcMF5, a pollen coat protein gene (PCP), from Brassica rapa. ssp. chinensis, involved in the transcription of different lengths of 3′-UTRs of PCPs
复制标题
BcMF5 是白菜花粉外壳蛋白基因 (PCP)，参与不同长度的 PCP 3-UTR 的转录。
DOI：
10.1007/s11033-007-9104-4
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发表时间：
2008
影响因子：
2.8
通讯作者：
Xiaolin Yu
中科院分区：
文献类型：
--
作者：
Qiang Zhang;Jiashu Cao;Li Huang;X. Xiang;Xiaolin Yu
在大白菜(Brassica Rapa L.SSP)野生型花蕾中积累了两个同源性较高的转录产物片段(GenBank登录号分别为DN237907.1和DN237908.1)。Chinensis Makino)进行了分离和研究。通过快速扩增cDNAEnds(RACE)获得两个片段的全长cDNAs。序列比对表明，它们除少数核苷酸有差异外，其余序列相同，应属于同一基因，即Brassica rapaMale Fertile5(BcMF5)BcMF5基因由252个核苷酸组成，编码一个83个氨基酸的蛋白质，并被一个256个核苷酸的内含子打断。序列BLAST分析表明，BcMF5是花粉外壳蛋白(PCP)基因家族的成员，与SLR-BP有很高的同源性。在3‘RACE过程中，从野生型MMC突变体中发现了8个不同长度的3’-UTR序列。Southern杂交分析表明，BcMF5在大白菜基因组中可能是一个单拷贝基因，这意味着8个不同长度的3‘-UTR序列可能来自同一个基因，可能是BcMF5的3’-UTRs的多个位点多聚腺苷化的结果。通过序列分析、Southern杂交结合RT-PCR和Northern杂交发现，BcMF5的3‘-UTRs含有一些功能元件，它们的时空表达模式不同，但都在野生型的IV期和V期花蕾中强烈表达。这表明在BcMF5转录过程中，不同长度的3‘-UTR可能参与了一种调控机制。
Two transcript-derived fragments (GenBank accession number DN237907.1 and DN237908.1) with high homology accumulated in the wild-type flower buds of Chinese cabbage (Brassica rapa L. ssp. chinensis Makino) are isolated and investigated. By rapid amplification of cDNA ends (RACE), the full length cDNA of the two fragments were obtained. The alignment of their cDNA sequence showed that they are identical except for differences in a few nucleotides and should belong to the same gene, namely, Brassica rapaMale Fertile5 (BcMF5). The BcMF5 gene consists of 252 bp encoding a protein of 83 amino acids and is interrupted by an intron of 256 bp. Sequence blast analysis revealed that BcMF5 is a member of the pollen coat protein (PCP) gene family and shared a high homology to SLR-BP. In the process of 3′RACE, eight different lengths of 3′-UTR sequence are found from the wild type of the mmc mutant. Southern blot analysis showed that BcMF5 could be a single-copy gene in the Chinese cabbage genome, implying that eight different lengths of 3′-UTR sequences might come from the same gene and could be a result of multiple sites polyadenylation of 3′-UTRs of BcMF5. Based on sequence analysis, southern hybridization combined with RT-PCR, and northern hybridization, it was discovered that 3′-UTRs of BcMF5 contained some functional elements and their temporal and spatial expression patterns were different, but all strongly expressed in the stage IV and stage V flower buds of wild type. This indicate that different lengths of 3′-UTR may be involved in a regulation mechanism during the transcription of BcMF5.