Molecular Epidemiology of Rotavirus in Central and Southeastern Europe
Molecular Epidemiology of Rotavirus in Central and Southeastern Europe
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中欧和东南欧轮状病毒的分子流行病学
DOI：
10.1128/jcm.00484-07
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发表时间：
2007
影响因子：
9.4
通讯作者：
L. Fiore
中科院分区：
文献类型：
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作者：
O. Tcheremenskaia;G. Marucci;Simona De Petris;F. Ruggeri;Darja Dovecar;S. L. Sternak;I. Matyasova;M. Dhimolea;Z. Mladenova;L. Fiore
摘要 罗马高级卫生研究所与捷克共和国、斯洛文尼亚、克罗地亚、阿尔巴尼亚和保加利亚的病毒学实验室合作实施了一个监测网络。 2004年至2006年间，从医院或门诊病房收治的重症胃肠炎患儿中采集了约1500份轮状病毒阳性粪便样本，通过逆转录巢式PCR测定G和P基因型。参与该研究的国家之间轮状病毒基因型的地理分布存在显着差异。 “常见”G/P 组合 G1P[8]、G3P[8]、G4P[8] 和 G2P[4] 的患病率在 50% 到 85% 之间。 G9 基因型在全球范围内出现，根据国家/地区的不同，所有样本中的 2% 至 35% 都被鉴定出。在 3% 至 20% 的患者样本中发现了不寻常的组合，例如 G1 或 G4 与 P[4] 相关或 G2 与 P[8] 相关，这些组合可能是由人类毒株之间的重配产生的。还鉴定出了可能源自动物的罕见基因型 G8P[8] 和 G10P[6]。在 1.7% 至 14% 的研究病例中观察到两种轮状病毒株的双重感染。我们的研究结果可能暗示了在巴尔干和中东欧广大地理区域实施轮状病毒疫苗所面临的挑战，并强调了广泛的毒株监测对于疫苗开发成功的重要性。
ABSTRACT A surveillance network was implemented by the Istituto Superiore di Sanità of Rome in collaboration with laboratories of virology in Czech Republic, Slovenia, Croatia, Albania, and Bulgaria. About 1,500 rotavirus-positive stool samples were collected from children with severe gastroenteritis admitted to hospitals or outpatient wards between 2004 and 2006. The G and P genotypes were determined by reverse transcription-nested PCR. Significant differences were found in the geographical distributions of rotavirus genotypes between countries participating in the study. The prevalence of “common” G/P combinations, G1P[8], G3P[8], G4P[8], and G2P[4], ranged between 50 and 85%. The G9 genotype, which is emerging worldwide, was identified in 2 to 35% of all samples depending on the country. Unusual combinations, such as G1 or G4 associated with P[4] or G2 with P[8], which may have arisen by reassortment between human strains, were found in samples from 3 to 20% of patients. The uncommon genotypes G8P[8] and G10P[6], which may have an animal origin, were also identified. Double infections with two rotavirus strains were observed in between 1.7 and 14% of cases studied. Our findings might implicate challenges for rotavirus vaccine implementation in a wide geographic area of the Balkans and Central-Eastern Europe and underscore the importance of extensive strain surveillance for success in vaccine development.
Matsumoto 等人：“副溶血性弧菌 O3:K6 克隆的大流行传播以及相关菌株的出现，由任意引物 PCR 和 toxRS 序列分析证明”J.
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尼日利亚分离的 G8 牛轮状病毒和人轮状病毒之间存在密切关系。
DOI：
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发表时间：
2003
期刊：
J Clin Microbiol 41(8)
影响因子：
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作者：
Adah MI;Nagashima S;Wakuda M;Taniguchi K
通讯作者：
Taniguchi K