Dual functional molecular imaging probe targeting CD20 with PET and optical imaging
Dual functional molecular imaging probe targeting CD20 with PET and optical imaging
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DOI：
10.3892/or_00000413
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发表时间：
2009-07-01
期刊：
影响因子：
4.2
通讯作者：
Endo, Keigo
中科院分区：
文献类型：
--
作者：
Paudyal, Pramila;Paudyal, Bishnuhari;Endo, Keigo
本研究旨在开发一种基于单克隆抗体（mAb）的双功能探针，用于靶向CD20的PET和近红外荧光（NIRF），并交叉验证该双功能探针的靶向功效。 NuB2（抗 CD20 mAb）通过 DOTA 螯合剂与 Alexa Fluor 750 和 (64)Cu 缀合。在 CD20 阳性 Raji 淋巴瘤小鼠中注射 (64)Cu-DOTA-NuB2-Alexa Fluor 750 后 30 分钟和 24 小时进行 PET 和 NIRF 成像。通过注射后 30 分钟、24 和 48 小时的离体生物分布研究来研究荧光强度和放射性。 Raji 肿瘤对 DOTA-NuB2-Alexa Fluor 750 的摄取显着高于 DOTA-Alexa Fluor 750 (p
The present study aimed to develop a monoclonal antibody (mAb)-based double functional probe for PET and near-infrared fluorescence (NIRF) targeting CD20 and to cross validate the targeting efficacy of this dual functional probe. NuB2, anti CD20 mAb was conjugated with Alexa Fluor 750 and (64)Cu through DOTA chelator. PET and NIRF imaging was carried out at 30 min and 24 h post-injection with (64)Cu-DOTA-NuB2-Alexa Fluor 750 in CD20 positive Raji lymphoma-bearing mice. Fluorescence intensity and radio-activity were studied by ex vivo biodistribution study at 30 min, 24 and 48 h after injection. Raji tumor showed significantly higher uptake of DOTA-NuB2-Alexa Fluor 750 than that of DOTA-Alexa Fluor 750 (p