Molecularly imprinted polymer based micromechanical cantilever sensor system for the selective determination of ciprofloxacin
Molecularly imprinted polymer based micromechanical cantilever sensor system for the selective determination of ciprofloxacin
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DOI：
10.1016/j.bios.2016.08.047
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发表时间：
2017-02-15
影响因子：
12.6
通讯作者：
Duman, Memed
中科院分区：
文献类型：
--
作者：
Okan, Meltem;Sari, Esma;Duman, Memed
本研究的主要目的是开发一种基于分子印迹聚合物（MIP）的微机械悬臂梁传感器系统，该系统具有高特异性、响应时间快、易于使用等特点，可用于水资源中环丙沙星（CPX）分子的检测。采用细乳液聚合技术合成了高特异性的CPX分子印迹纳米粒子。合成的纳米粒子的平均尺寸约为160 nm，具有较高的单分散性。通过EDC/NHS活化提供MIP纳米颗粒在杠杆上的共价和单层结合。在空气中进行了验证悬臂梁纳米传感器的浸渍和干燥技术，通过采用动态传感模式。根据所获得的结果，微机械悬臂梁传感器系统在1.5-150.9 μ M的浓度范围内线性工作。该浓度范围导致MIP修饰的悬臂上的18.4-48.9 pg质量负载。所开发的传感器的灵敏度计算为2.6 Hz/pg。为了控制MIP的特异性，使用具有与CPX相似的物理和化学结构的不同抗生素恩诺沙星（ENF），其显示7倍低的结合亲和力。开发的高度特定的微悬臂梁传感器具有约2分钟的响应时间，可重复使用多达4次。结果表明，基于MIP的AFM纳米传感器对CPX分子具有较高的灵敏度。MIP纳米颗粒与微机械传感器的这种组合是质量传感应用中的先驱研究之一。本研究开发的这种快速，低成本和高灵敏度的CPX特异性MIP纳米颗粒为基础的纳米传感器有可能为进一步的研究铺平道路。(C)2016爱思唯尔B. V.保留所有权利。
The main objective of this study is to develop molecularly imprinted polymer (MIP) based micro mechanical cantilever sensor system that has high specificity, fast response time and is easily applicable by user for the detection of ciprofloxacin (CPX) molecule in water resources. Highly specific CPX imprinted nanoparticles were synthesized by miniemulsion polymerization technique. The average size of the synthesized nanoparticles was measured about 160 nm with high monodispersivity. Covalent and monolayer binding of the MIP nanoparticles on cantilevers was provided by EDC/NHS activation. Validation of the developed cantilever nanosensor was performed in air with dip-and-dry technique by employing the dynamic sensing mode. According to the results obtained, micromechanical cantilever sensor system worked linearly for the concentration range of 1.5-150.9 mu M. This concentration range resulted with 18.4-48.9 pg mass load on the MIP modified cantilever. The sensitivity of the developed sensor was calculated as 2.6 Hz/pg. To control the specificity of MIPs, a different antibiotic enrofloxacin (ENF), with a similar physical and chemical structure with CPX, was used, which showed 7 folds low binding affinity. The developed highly specific microcantilever sensor has a response time of approximately 2 min and is reusable up to 4 times. The results indicate that the MIP based AFM nanosensor has high sensitivity for the CPX molecule. This combination of MIP nanoparticles with micromechanical sensors is one of the pioneer studies in the mass sensing applications. This fast, low cost and highly sensitive CPX specific MIP nanoparticle based nanosensor developed in this research have the potential to pave the way for further studies. (C) 2016 Elsevier B.V. All rights reserved.