A flagella-less mutant of Borrelia burgdorferi. Structural, molecular, and in vitro functional characterization.
A flagella-less mutant of Borrelia burgdorferi. Structural, molecular, and in vitro functional characterization.
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伯氏疏螺旋体的无鞭毛突变体。
DOI：
10.1172/jci115308
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Barbour,AG
中科院分区：
文献类型：
--
作者：
Sadziene,A;Thomas,DD;Bundoc,VG;Holt,SC;Barbour,AG
分离并鉴定了莱姆病病原体伯氏疏螺旋体的非运动突变体。将突变体与野生型前体以及具有相同遗传背景的能动回复回复体进行比较。根据形态学、生化和免疫学标准，该突变体缺乏鞭毛的主要结构蛋白鞭毛蛋白。该突变与主要 DNA 重排或转录失败无关。鞭毛缺失的一个明显后果是体外穿透人内皮细胞层的能力降低。在另一项功能意义评估中，当用酶联免疫吸附试验检测抗 B 型杆菌时，无鞭毛的突变体即使不优于带鞭毛的同基因分离株，也是相同的。莱姆病患者血清中存在伯氏杆菌抗体。这些突变体的研究是致病性疏螺旋体属中的第一个。待表征表明，鞭毛及其赋予的运动性在伯氏疏螺旋体入侵人体组织中发挥作用。无鞭毛的伯氏疏螺旋体可能可用作更特异性免疫测定和预防莱姆病疫苗的基础。图像
A nonmotile mutant of Borrelia burgdorferi, the etiologic agent of Lyme disease, was isolated and characterized. The mutant was compared with the wild-type predecessor as well as with a motile back-revertant of the same genetic background. The mutant lacked, by morphologic, biochemical, and immunologic criteria, the major structural protein of flagella, flagellin. This mutation was not associated with major DNA rearrangements or with failure of transcription. An apparent consequence of a loss of flagella was reduced ability to penetrate human endothelial cell layers in vitro. In another assessment of functional significance, the flagella-less mutant was equal if not superior to flagella-bearing, isogenic isolates when examined in an enzyme-linked immunosorbent assay for anti-B. burgdorferi antibodies in the sera of Lyme disease patients. These studies of a mutant, the first among pathogenic Borrelia spp. to be characterized, indicate that the flagellum and motility it confers play a role in B. burgdorferi's invasion of human tissues. A flagella-less B. burgdorferi may be useful as the basis of a more specific immunoassay and a vaccine for protection against Lyme disease.Images