The use of potassium permanganate as an electron-dense stain for sections of tissue embedded in epoxy resin.
The use of potassium permanganate as an electron-dense stain for sections of tissue embedded in epoxy resin.
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DOI：
10.1083/jcb.7.1.197
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发表时间：
1960-02
期刊：
影响因子：
--
通讯作者：
LAWN AM
中科院分区：
文献类型：
--
作者：
LAWN AM
高锰酸钾已成为电子显微镜中常用的固定剂（Luft, 1956），在细胞膜研究中具有特殊的价值。在研究提高环氧树脂包埋组织在电子显微镜下对比度的方法时，使用高锰酸钾溶液处理切片，使用类似于Watson（1958）的技术。发现高锰酸钾会产生明显的对比度增加，但这通常伴随着切片表面电子不透明物质的粒状沉淀。结果表明，柠檬酸的弱溶液可以去除这种沉淀物，而不会严重降低切片的对比度。建议以下方法作为进一步改进的起点。
Potassium permanganate has become an established fixative in electron microscopy (Luft, 1956) and has special value in the study of cell membranes. During an investigation of methods of increasing the contrast in the electron microscope of tissues embedded in epoxy resin, ta solution of potassium permanganate was used to treat the sections, using a technique similar to that of Watson (1958). Potassium permanganate was found to produce a marked increase in contrast, but this was usually accompanied by a granular precipitate of electron-opaque material on the surface of the section. It was found that a weak solution of citric acid could be used to remove this precipitate without seriously reducing the contrast of the section. The following method is suggested as a starting point for further improvement.