Incorrect identification of recent HIV infection in adults in the United States using a limiting-antigen avidity assay
Incorrect identification of recent HIV infection in adults in the United States using a limiting-antigen avidity assay
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DOI：
10.1097/qad.0000000000000221
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发表时间：
2014-05-15
期刊：
影响因子：
3.8
通讯作者：
Laeyendecker, Oliver
中科院分区：
文献类型：
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作者：
Longosz, Andrew F.;Mehta, Shruti H.;Laeyendecker, Oliver
目的：为了评估与错误分类的限制性抗原亲合力（LAG亲合力）测定长期HIV infection.Design的个人之间的相关因素：样品获得了多中心艾滋病队列研究和艾滋病与静脉内经验队列（1089样本667个人，595样本收集2-4年和494样本收集4-8年后，艾滋病毒血清转化）。两个时间点的配对样本可用于422（63.3%）的667 individuals.Methods：如果LAg-亲合力检测结果为1.5或更低的归一化光密度（OD-n）units.Results：总体而言，4.8%（52/1089）的样本被错误分类，包括1.8% [16/884，95%置信区间（CI）1.09-3.06%]的病毒载量超过400拷贝/ml的个体样本和1.4%（10/705）的病毒载量超过400拷贝/ml和CD 4（+）细胞计数超过200个细胞/μ l（95%CI 0.68-2.60%）的个体样本。年龄、种族、性别和HIV感染模式与错误分类无关。在校正分析中，病毒载量低于400拷贝/ml [校正比值比（aOR）3.72，95% CI 1.61-8.57]，CD 4（+）细胞计数低于50个细胞/μ l（aOR 5.41，95% CI 1.86-15.74），低LAg亲合力结果（
Objectives:To evaluate factors associated with misclassification by the limiting-antigen avidity (LAg-avidity) assay among individuals with long-standing HIV infection.Design:Samples were obtained from the Multicenter AIDS Cohort Study and AIDS Linked to the IntraVenous Experience cohort (1089 samples from 667 individuals, 595 samples collected 2-4 years and 494 samples collected 4-8 years after HIV seroconversion). Paired samples from both time points were available for 422 (63.3%) of the 667 individuals.Methods:Samples were considered to be misclassified if the LAg-avidity assay result was 1.5 or less normalized optical density (OD-n) units.Results:Overall, 4.8% (52/1089) of the samples were misclassified, including 1.8% [16/884, 95% confidence interval (CI) 1.09-3.06%] of samples from individuals with viral loads above 400 copies/ml and 1.4% (10/705) of samples from individuals with viral loads above 400 copies/ml and CD4(+) cell counts above 200 cells/mu l (95% CI 0.68-2.60%). Age, race, sex, and mode of HIV acquisition were not associated with misclassification. In an adjusted analysis, viral load below 400 copies/ml [adjusted odds ratio (aOR) 3.72, 95% CI 1.61-8.57], CD4(+) cell count below 50 cells/mu l (aOR 5.41, 95% CI 1.86-15.74), and low LAg-avidity result (