The effect of dipotassium EDTA and lithium heparin on hematologic values of farmed brown trout Salmo trutta (L.) spawners
The effect of dipotassium EDTA and lithium heparin on hematologic values of farmed brown trout Salmo trutta (L.) spawners
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DOI：
10.1007/s10499-018-0308-5
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发表时间：
2019-02-01
影响因子：
2.9
通讯作者：
Jerzak, Leszek
中科院分区：
文献类型：
--
作者：
Cieplinski, Mateusz;Kasprzak, Mariusz;Jerzak, Leszek
血液分析是动物健康和福利控制中非常重要和强大的诊断工具。它通常在高等脊椎动物中进行，其参考值已经很好地建立，但鱼类血液学仍需进一步研究。许多内在因素和环境因素对鱼类血液学值有着深刻的影响，给参考值的确定带来困难。此外，由于凝血时间短，鱼血通常需要添加抗凝剂。抗凝剂的选择对于获得可靠的血液检测值至关重要。在本研究中，两种常见的抗凝剂，K（2）EDTA（1.8 mg/ml）和肝素锂（18 I.U./ ml），对养殖褐鳟（萨尔莫trutta）产卵期血液学指标的影响。比较了这两种化合物的基本血液学分析结果，如红细胞压积（PCV）、血红蛋白浓度（HGB）、红细胞计数（RBC）、平均红细胞血红蛋白（MCH）、平均红细胞体积（MCV）、平均红细胞血红蛋白浓度（MCHC）和白色血细胞计数（WBC）。在PCV、MCV和MCHC中观察到统计学显著性差异，而HGB、RBC、MCH和WBC未显示此类差异。这些结果表明，肝素锂给出了更可靠的结果，因为K（2）EDTA处理的样本中的红细胞有膨胀的趋势。值得注意的是，乙二胺四乙酸盐在本研究中未诱导样品溶血。
Blood analysis is a very important and powerful diagnostic tool in animal health and welfare control. It is routinely performed in higher vertebrates, for which reference values are well established, but fish hematology still needs further research. Many intrinsic and environmental factors have profound impact on fish hematological values, making determination of reference values difficult. Additionally, fish blood usually requires the addition of an anticoagulant agent, because of short clotting times. The choice of anticoagulant is vital for obtaining reliable blood test values. In the present study, the impact of two common anticoagulants, K(2)EDTA (1.8 mg/ml) and lithium heparin (18 I.U./ml), on hematological values of farmed brown trout Salmo trutta spawners during the spawning season was investigated. Results of basic hematological analysis, such as packed cell volume (PCV), hemoglobin concentration (HGB), red blood cell count (RBC), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and white blood cell count (WBC), were compared between these two compounds. Statistically significant differences were observed in PCV, MCV, and MCHC, whereas HGB, RBC, MCH, and WBC showed no such differences. These results suggest that lithium heparin gives more reliable results, because red blood cells in K(2)EDTA-treated samples have a tendency to swell. It is worth noting that ethylenediaminetetraacetic acid salt did not induce sample hemolysis in the present study.