Lectin from sainfoin (Onobrychis viciifolia scop.). Complete amino acid sequence.

Lectin from sainfoin (Onobrychis viciifolia scop.). Complete amino acid sequence.
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来自红豆甙(Onobrychis viciifolia scop.)的凝集素。

DOI:
10.1021/bi00303a038
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发表时间:
1984
期刊:
影响因子:
2.9
通讯作者:
Hapner,KD
Hapner,KD
中科院分区:
生物学3区
文献类型:
--
作者:
Kouchalakos,RN;Bates,OJ;Bradshaw,RA;Hapner,KD

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材料和方法蛋白质制备。红豆草凝集素是从红豆草种子中分离出来的(R. L. Ditterline,Montana州立大学),并对以前的方法进行了轻微的修改(Hapner & Robbins 1979)。初始提取步骤在D-葡萄糖中为0.1 M,并省略硫酸铵分级步骤。通过过滤和离心除去不溶性碎片,并以分批方式将凝集素吸附到亲和基质上。D-葡萄糖从化学系和蒙大拿农业实验站,蒙大拿州博兹曼的蒙大拿州立大学,蒙大拿州59717(RNK和KDH)和生物化学系,医学院,华盛顿大学,圣路易斯,密苏里州63110(OJB和RAB)中去除。1983年8月22日收到。这项工作得到了美国农业部(616-15-76)和美国公共卫生署(AM 13362)的赠款,并作为蒙大拿州农业实验站的J 1449号论文发表。初步来文已经出现(Kouchalakos和Hapner,1982年)。
Materials and Methods Protein Preparation. Sainfoin lectin was isolated from ground sainfoin seeds (gift from R. L. Ditterline, Montana State University) with a slight modification of theprevious procedure (Hapner & Robbins 1979). The initial extraction step was made 0.1 M in D-glucose, and the ammonium sulfate fractionation step was omitted. Insoluble debris was removed by filtration and centrifugation, and the lectin was absorbed to the affinity matrix in batch fashion. D-Glucose was removed f From the Department of Chemistry and Montana Agricultural Experiment Station, Montana State University, Bozeman, Montana 59717 (RNK and KDH), and theDepartment of Biological Chemistry, School of Medicine, Washington University, St. Louis, Missouri 63110 (OJB and RAB). Received August 22, 1983. This work was sup-ported in part by grants from the US Department of Agriculture (616-15-76) and the US Public Health Service (AM 13362) and is published as Paper No. J 1449 from the Montana Agricultural Experi-ment Station. A preliminary communication has appeared (Kouchalakos & Hapner, 1982).
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