A genetic map and recombination parameters of the human malaria parasite Plasmodium falciparum
A genetic map and recombination parameters of the human malaria parasite Plasmodium falciparum
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DOI：
10.1126/science.286.5443.1351
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发表时间：
1999-11-12
期刊：
影响因子：
56.9
通讯作者：
Wellems, TE
中科院分区：
文献类型：
--
作者：
Su, XZ;Ferdig, MT;Wellems, TE
疟疾的遗传学研究需要一个全基因组的，高分辨率的恶性疟原虫连锁图谱。遗传杂交用于从901个标记构建这样的图谱，所述标记落入对应于14条核染色体的14个推断的连锁群。染色体组中的减数分裂交换活性被证明是高的（每厘摩17个减数分裂酶），并且在染色体长度上显著均匀。基因转换事件和自发的微卫星长度的变化是显而易见的遗传数据。这些标记、图谱和重组参数有助于基因组序列的组装、毒力和抗药性等性状的决定因素的定位以及寄生虫田间种群的遗传研究。
Genetic investigations of malaria require a genome-wide, high-resolution Linkage map of Plasmodium falciparum. A genetic cross was used to construct such a map from 901 markers that fall into 14 inferred Linkage groups corresponding to the 14 nuclear chromosomes. Meiotic crossover activity in the genome proved high (17 kilobases per centimorgan) and notably uniform over chromosome length. Gene conversion events and spontaneous microsatellite length changes were evident in the inheritance data. The markers, map, and recombination parameters are facilitating genome sequence assembly, Localization of determinants for such traits as virulence and drug resistance, and genetic studies of parasite field populations.