Cell cycle arrest and apoptosis in Caenorhabditis elegans germline cells following heavy-ion microbeam irradiation
Cell cycle arrest and apoptosis in Caenorhabditis elegans germline cells following heavy-ion microbeam irradiation
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DOI：
10.1080/09553000600577821
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发表时间：
2006-01-01
影响因子：
2.6
通讯作者：
Higashitani, A
中科院分区：
文献类型：
--
作者：
Sugimoto, T;Dazai, K;Higashitani, A
目的：利用实验模型后生动物秀丽隐杆线虫，研究高能重离子微束辐射对生殖细胞的位置影响。材料和方法：用从方位角变化场（AVF）回旋加速器发射的220MeV C-12（5+）粒子的光栅扫描宽光束或准直微束照射生殖细胞，随后观察细胞周期停滞和细胞周期停滞。细胞凋亡。结果：在 L4 幼虫阶段用宽光束进行全身照射可抑制生殖细胞增殖。当在L4阶段用微束局部照射性腺臂的尖端区域时，观察到相同的停滞。当微束用于照射性腺臂的粗线期区域时，在年轻妊娠阶段，性腺中发生了辐射诱导的细胞凋亡。相反，在未受辐射的邻近区域或相对的性腺中没有诱导停滞和细胞凋亡。类似的结果在对辐射诱导的细胞凋亡过敏的 c-abl-1（Abelson 鼠白血病病毒的细胞对应物的哺乳动物直系同源物）突变体中得到证实。结论：这些结果表明微束照射可用于表征生物体对辐射的组织特异性、局部生物反应。在局部照射区域观察到 DNA 损伤诱导的细胞周期停滞和细胞凋亡，但在线虫中几乎没有“旁观者效应”。
Purpose: To investigate positional effects of radiation with an energetic heavy-ion microbeam on germline cells using an experimental model metazoan Caenorhabditis elegans.Materials and methods: The germline cells were irradiated with raster-scanned broad beam or collimated microbeam of 220MeV C-12(5+) particles delivered from the azimuthally varying field (AVF) cyclotron, and subsequently observed for cell cycle arrest and apoptosis.Results: Whole-body irradiation with the broad beam at the L4 larval stage arrested germ cell proliferation. When the tip region of the gonad arm was irradiated locally with the microbeam at the L4 stage, the same arrest was observed. When the microbeams were used to irradiate the pachytene region of the gonad arm, at a young gravid stage, radiation-induced apoptosis occurred in the gonad. In contrast, arrest and apoptosis were not induced in the non-irradiated neighboring region or the opposite gonad. Similar results were confirmed in the c-abl-1 (mammalian ortholog of cellular counterpart of Abelson murine leukemia virus) mutant that is hypersensitive to radiation-induced apoptosis.Conclusion: These results indicate that the microbeam irradiation is useful in characterizing tissue-specific, local biological response to radiation in organisms. DNA damage-induced cell cycle arrest and apoptosis were observed in locally irradiated regions, but there was little, if any, 'bystander effect' in the nematode.