Thyroid hormone decreases thyrotropin subunit mRNA levels in rat anterior pituitary.
Thyroid hormone decreases thyrotropin subunit mRNA levels in rat anterior pituitary.
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甲状腺激素降低大鼠垂体前叶促甲状腺素亚基 mRNA 水平。
DOI：
10.1089/dna.1.1984.3.231
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Maurer,RA
中科院分区：
文献类型：
--
作者：
Croyle,ML;Maurer,RA
含有大鼠促甲状腺激素亚基序列的重组DNA克隆已被分离、鉴定，并用于研究亚基mRNA水平的激素调节。构建了克隆在质粒pBR 322中的大鼠cDNA序列文库，并使用先前鉴定的克隆牛cDNA筛选促甲状腺素序列。大鼠促甲状腺激素亚基cDNA的身份通过核苷酸序列分析证实。然后将克隆的cDNA用作杂交探针，以检测三碘甲状腺原氨酸对促甲状腺激素α和β亚基mRNA水平的影响。结果表明，甲状腺激素处理降低大鼠垂体前叶α和β亚基mRNA的水平。
Recombinant DNA clones containing sequences for the subunits of rat thyrotropin have been isolated, characterized, and used to examine the hormonal regulation of subunit mRNA levels. A library of rat cDNA sequences cloned in plasmid pBR322 was constructed and screened for thyrotropin sequences using cloned bovine cDNAs which had been previously identified. The identity of rat thyrotropin subunit cDNAs was confirmed by nucleotide sequence analysis. The cloned cDNAs were then used as hybridization probes to examine the effects of triiodothyronine on the levels of the α- and β-subunit m RNAs of thyrotropin. The results demonstrate that treatment with thyroid hormone decreases the levels of both α- and β-subunit mRNA in rat anterior pituitary.