The differential expression of hepatocyte growth factor and met in human placenta.
The differential expression of hepatocyte growth factor and met in human placenta.
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DOI：
10.1210/jcem.82.3.3806
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发表时间：
1997-03
期刊：
影响因子：
--
通讯作者：
S. Kauma;N. Hayes;S. Weatherford
中科院分区：
文献类型：
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作者：
S. Kauma;N. Hayes;S. Weatherford
Met是肝细胞生长因子（HGF）配体的酪氨酸激酶受体。Met/HGF在上皮细胞增殖、迁移和形态发生中起重要作用。HGF在小鼠胎盘发育中也起着至关重要的作用。为了确定HGF是否在人类胎盘发育中具有类似的作用，在妊娠早期和足月胎盘中测定了Met和HGF的产生和定位。逆转录-聚合酶链反应（RT-PCR）显示胎盘绒毛组织中有Met和HGF信使核糖核酸的表达。用酶联免疫吸附试验测定肝细胞生长因子的产生。培养中的孕中期胎盘绒毛外植体在48小时内的HGF产量（810 pg/mg总蛋白x h）是足月胎盘绒毛外植体的2.1倍（380 pg/mg总蛋白x h; P < 0.01）。分离的滋养层细胞不产生HGF，而分离的绒毛核心组织和绒毛核心间充质细胞产生HGF。白细胞介素-1 β处理胎盘绒毛或绒毛核心间充质细胞与分离的滋养层细胞共培养没有刺激HGF的产生。使用免疫组化，肝细胞生长因子定位于绒毛核心区室，没有定位到滋养层。相反，Met主要定位于细胞滋养层。这些发现表明，绒毛核心产生的HGF可能通过HGF受体Met以旁分泌方式调节滋养层细胞的发育或功能。
Met is the tyrosine kinase receptor for the ligand hepatocyte growth factor (HGF). Met/HGF plays an important role in epithelial cell proliferation, migration, and morphogenesis. HGF also plays a crucial role in placental development in the mouse. To determine whether HGF potentially has a similar role in human placental development, the production and localization of Met and HGF were determined in early second trimester and term placentas. Reverse transcription-PCR using specific primers demonstrated the expression of Met and HGF messenger ribonucleic acid in placental villi. HGF production was determined by enzyme-linked immunosorbent assay. HGF production over 48 h by second trimester placental villous explants in culture (810 pg/mg total protein x h) was 2.1-fold greater than that in term placental villous explants (380 pg/mg total protein x h; P < 0.01). Isolated trophoblast did not produce HGF, whereas isolated villous core tissues and villous core mesenchymal cells did produce HGF. Interleukin-1 beta treatment of placental villi or coculture of villous core mesenchymal cells with isolated trophoblast cells did not stimulate HGF production. Using immunohistochemistry, HGF localized to the villous core compartment with no localization to the trophoblast. In contrast, Met localized mainly to cytotrophoblast. These findings suggest that HGF produced by the villous core may act in a paracrine fashion to regulate trophoblast development or function through the HGF receptor, Met.