Malate dehydrogenase: distribution, function and properties.
Malate dehydrogenase: distribution, function and properties.
复制标题
苹果酸脱氢酶：分布、功能和特性。
DOI：
--
复制
发表时间：
1998
影响因子：
1.5
通讯作者：
D. Mikulášová
中科院分区：
文献类型：
--
作者：
Rabia Ahmed Musrati;M. Kollarova;N. Mernik;D. Mikulášová
苹果酸脱氢酶 (MDH) (EC 1.1.1.37) 催化草酰乙酸和苹果酸的转化。该反应在细胞代谢中很重要，并且与易于检测的辅因子氧化/还原相结合。它是一种相当普遍的酶，已鉴定出多种亚型，其亚细胞定位及其对辅因子 NAD 或 NADP 的特异性不同。核苷酸结合特性可以通过单个氨基酸的变化来改变。 MDH 的多个氨基酸序列比对表明，除了对催化、辅因子结合和亚基界面至关重要的几个位置之外，一级结构相似性较低。尽管氨基酸序列同一性较低，但它们的 3 维结构非常相似。 MDH 是一组由相同亚基组成的多聚酶，通常组织为二聚体或四聚体，亚基分子量为 30-35 kDa。 MDH 已从不同来源中分离出来，包括古细菌、真细菌、真菌、植物和哺乳动物。
Malate dehydrogenase (MDH) (EC 1.1.1.37) catalyzes the conversion of oxaloacetate and malate. This reaction is important in cellular metabolism, and it is coupled with easily detectable cofactor oxidation/reduction. It is a rather ubiquitous enzyme, for which several isoforms have been identified, differing in their subcellular localization and their specificity for the cofactor NAD or NADP. The nucleotide binding characteristics can be altered by a single amino acid change. Multiple amino acid sequence alignments of MDH show that there is a low degree of primary structural similarity, apart from several positions crucial for catalysis, cofactor binding and the subunit interface. Despite the low amino acids sequence identity their 3-dimensional structures are very similar. MDH is a group of multimeric enzymes consisting of identical subunits usually organized as either dimer or tetramers with subunit molecular weights of 30-35 kDa. MDH has been isolated from different sources including archaea, eubacteria, fungi, plant and mammals.
影响因子：
5.6
作者：
GRAU, UM;TROMMER, WE;ROSSMANN, MG
通讯作者：
ROSSMANN, MG