Mechanisms of cell reproductive death and shapes of radiation dose--survival curves of mammalian cells.
Mechanisms of cell reproductive death and shapes of radiation dose--survival curves of mammalian cells.
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细胞生殖死亡的机制和辐射剂量的形状——哺乳动物细胞的生存曲线。
DOI：
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发表时间：
1990
影响因子：
2.6
通讯作者：
G. Barendsen
中科院分区：
文献类型：
--
作者：
G. Barendsen
提出了电离辐射导致细胞生殖死亡的“配对dsb”作用机制，该机制允许解释由辐射的线性能量转移变化、细胞周期阶段、细胞培养条件以及对化合物的敏感和保护引起的存活曲线形状差异。这是基于对生存曲线形状的S(D)/S(O) = exp - (a1D + a2D2)的分析，以及在10纳米量级的距离内产生的DNA中成对的dsb在启动单个粒子轨迹导致细胞生殖死亡的事件序列方面是有效的。部分致命性可能来自单轨在较远距离上产生的两个dsb，这可能构成潜在的致命损害，但在有利条件下可以修复。因此，存活曲线的初始斜率并非与细胞的修复能力无关，而是可以被细胞条件所改变。在生存方程中造成二次项的损害可以解释为由两个不同的电离粒子产生的两个dsb的结果，尽管不能排除其他相互作用。“配对dsb”损伤的机制与细胞及其成分中不同作用对LET的依赖性的信息是一致的。
A 'paired dsb' mechanism of action for cell reproductive death by ionizing radiations is proposed, which allows interpretation of differences in shapes of survival curves caused by variation of linear energy transfer of the radiation, by the stage in the cell cycle, but cell culture conditions and by sensitizing and protecting compounds. It is based on the analysis of shapes of survival curves in terms of S(D)/S(O) = exp - (a1D + a2D2) and the suggestion that paired dsb in DNA, produced within distances of the order of 10 nm, are efficient in initiating the sequence of events causing cell reproductive death by individual particle tracks. Part of the lethality may result from two dsb's produced by single tracks at larger distances, and this might constitute potentially lethal damage which in favourable conditions can be repaired. Thus, the initial slope of a survival curve is not independent of the repair capacity of a cell, but indeed can be modified by cell conditions. The damage causing the quadratic term in the survival equation may be interpreted as a consequence of two dsb produced by two different ionizing particles, although other interactions cannot be excluded. The suggested mechanism of 'paired dsb' damage is consistent with information concerning the LET dependence of different effects in cells and their constituents.
影响因子：
3.4
作者：
CURTIS, SB
通讯作者：
CURTIS, SB