Simultaneous orientation and cellular force measurements in adult cardiac myocytes using three-dimensional polymeric microstructures.
Simultaneous orientation and cellular force measurements in adult cardiac myocytes using three-dimensional polymeric microstructures.
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DOI：
10.1002/cm.20218
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发表时间：
2007-09
影响因子：
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通讯作者：
Yi Zhao;C. Lim;D. Sawyer;R. Liao;Xin Zhang
中科院分区：
文献类型：
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作者：
Yi Zhao;C. Lim;D. Sawyer;R. Liao;Xin Zhang
已经开发了许多技术来监测离体心肌细胞的收缩功能。虽然从这些方法中获得了宝贵的观察结果，以了解心脏的收缩过程，但它们总是受到体外条件的限制。目前的挑战是开发创新的检测方法来模拟体内环境，以便能够对心肌细胞收缩力进行更生理学的评估。在这里，我们展示了使用硅橡胶弹性体，聚（二甲基硅氧烷）（PDMS），同时定向成人心肌细胞在原代培养和测量细胞的力量在一个三维基板。由于肌原纤维沿着平行的聚合物侧壁定向重组，在长期培养（7天）中实现了成人心肌细胞的重新排列。通过观察嵌入基底中的微柱的变形来原位记录细胞的机械力。通过将细胞机械力测量与芯片上细胞取向相结合，这种新的测定有望提供一种对单个心肌细胞收缩功能进行更生理评估的方法，并可能促进体外组装功能性心脏组织的未来发展。
A number of techniques have been developed to monitor contractile function in isolated cardiac myocytes. While invaluable observations have been gained from these methodologies in understanding the contractile processes of the heart, they are invariably limited by their in vitro conditions. The present challenge is to develop innovative assays to mimic the in vivo milieu so as to allow a more physiological assessment of cardiac myocyte contractile forces. Here we demonstrate the use of a silicone elastomer, poly(dimethylsiloxane) (PDMS), to simultaneously orient adult cardiac myocytes in primary culture and measure the cellular forces in a three-dimensional substrate. The realignment of adult cardiac myocytes in long-term culture (7 days) was achieved due to directional reassembly of the myofibrils along the parallel polymeric sidewalls. The cellular mechanical forces were recorded in situ by observing the deformation of the micropillars embedded in the substrate. By coupling the cellular mechanical force measurements with on-chip cell orientation, this novel assay is expected to provide a means of a more physiological assessment of single cardiac myocyte contractile function and may facilitate the future development of in vitro assembled functional cardiac tissue.